Author, as appears in the article.: Rehues, P; Rodríguez, M; Alvarez, J; Jiménez, M; Melià, A; Sempere, M; Balsells, C; Castillejo, G; Guardiola, M; Castro, A; Ribalta, J
Department: Medicina i Cirurgia
URV's Author/s: Alvarez Vicente, Juan Jose / Balsells Llauradò, Clara / Castillejo De Villasante, Gemma / Castro Salomó, Antoni / Guardiola Guionnet, Montserrat / Jiménez Herrera, María Francisca / Rehues Masip, Pere / Ribalta Vives, Josep / Rodríguez Chacón, Matilde
Keywords: Lipoprotein Lipopolysaccharide Atherosclerosis Adipose-tissue 3-hydroxy fatty acids plasma lipoprotein lipopolysaccharide kinetics expression endotoxin cholesterol binding atherosclerosis association 3-hydroxy fatty acids
Abstract: Atherosclerosis is a chronic inflammatory disease that is caused by the accumulation of LDL particles in the intima, causing the activation of immune cells and triggering an inflammatory response. LPS is a potent activator of the innate immune response and it can be transported by lipoproteins. Since humans are much more sensitive to LPS than other mammals, and very low amounts of LPS can elicit an immune response, the aim of this study is to characterize the distribution of LPS and its immunogenic portion (3OHFAs) among lipoprotein types of healthy men. We separated lipoprotein fractions by ultracentrifugation and the amount of each 3OHFA was measured by MS in each lipoprotein fraction to calculate LPS concentration. Lipoprotein particle concentration was measured by NMR. LDL and HDL fractions transported the highest concentration of LPS (35.7% and 31.5%, respectively), but VLDL particles carried more LPS molecules per particle (0.55 molecules/particle) than LDL or HDL (p < 0.01). The distribution of LPS and all 3OHFAs among lipoprotein fractions showed high interindividual variability, suggesting that they may be studied as a potential biomarker. This may help understand the role of LPS in atherosclerosis in those cases where the disease cannot be explained by traditional risk factors. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: pere.rehues@urv.cat gemma.castillejo@urv.cat matilde.rodriguez@urv.cat juanjose.alvarez@estudiants.urv.cat pere.rehues@urv.cat antoni.castro@urv.cat montse.guardiola@urv.cat maria.jimenez@urv.cat josep.ribalta@urv.cat
Author identifier: 0000-0001-5441-6333 0000-0002-9696-7384 0000-0003-2599-3742 0000-0002-8879-4719
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2218-273X/12/1/47
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Biomolecules. 12 (1):
APA: Rehues, P; Rodríguez, M; Alvarez, J; Jiménez, M; Melià, A; Sempere, M; Balsells, C; Castillejo, G; Guardiola, M; Castro, A; Ribalta, J (2022). Characterization of the lps and 3ohfa contents in the lipoprotein fractions and lipoprotein particles of healthy men. Biomolecules, 12(1), -. DOI: 10.3390/biom12010047
Article's DOI: 10.3390/biom12010047
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications