Serum lysophospholipidome of dietary origin as a suitable susceptibility/risk biomarker of human hypercholesterolemia: A cross-sectional study

Author, as appears in the article.: Calderon-Perez, Lorena; Suarez-Garcia, Susana; Pedret, Anna; Suarez, Manuel; Llaurado, Elisabet; Rubio, Laura; del Bas, Josep M; Caimari, Antoni; Puiggros, Francesc; Arola, Lluis; Sola, Rosa; Valls, Rosa M

Department: Medicina i Cirurgia; Bioquímica i Biotecnologia

URV's Author/s: Arola Ferrer, Luis Maria / Del Bas Prior, José María / Llauradó Ribé, Elisabet / Pedret Figuerola, Anna / Solà Alberich, Rosa Maria / SUÁREZ GARCÍA, SUSANA / Suárez Recio, Manuel

Keywords: Trimethyloxamine; Trimethylamine-n-oxide; Risk assessment; Polyunsaturated fatty acids; Methylamines; Metabolome; Lysophospholipids; Lysophosphatidylethanolamines; Lysophosphatidylethanolamine; Lysophosphatidylcholines; Liver; Hypercholesterolemia; Humans; Fatty liver; Eating; Disease susceptibility; Disease progression; Dietary fats; Diet; Cross-sectional studies; Cricetinae; Cholesterol, ldl; Biomarkers; Animals

Abstract: Background & aims: Whether bioactive lysophospholipids (lyso-PLs) and trimethylamine-N-oxide (TMAO) serve as non-invasive biomarkers in early human hypercholesterolemia (HC) is unknown. This study aimed to assess whether serum lyso-PLs and plasma TMAO may be suitable susceptibility/risk biomarkers of HC in humans. Secondarily, we aimed to evaluate the relationships between targeted metabolites, diet composition and circulating liver transaminases, and verify these results in hamsters. Methods: A targeted metabolomics and lipidomics approach determined plasma TMAO and serum lysophosphatidylcholines (lyso-PCs) and lysophosphatidylethanolamines (lyso-PEs) in low (L-LDL-c) and moderate to high (MH-LDL-c) LDL-cholesterol subjects. Additionally, the relationships between targeted metabolites, liver transaminases and diet, particularly fatty acid intake, were tested. In parallel, plasma and liver lyso-PL profiles were studied in 16 hamsters fed a moderate high-fat (HFD) or low-fat (LFD) diet for 30 days. Results: Predictive models identified lyso-PC15:0 and lyso-PE18:2 as the most discriminant lyso-PLs among groups. In MH-LDL-c (n = 48), LDL-cholesterol and saturated FAs were positively associated with lyso-PC15:0, whereas in L-LDL-c (n = 70), LDL-cholesterol and polyunsaturated fatty acids (PUFAs) were negatively and positively related to lyso-PE18:2, respectively. Interestingly, in MH-LDL-c, the lower lyso-PE 18:2 concentrations were indicative of higher LDL-cholesterol levels. Intrahepatic accumulation of lyso-PLs-containing essential n-6 PUFAs, including lyso-PE18:2, were higher in HFD-fed hamsters than LFD-fed hamsters. Conclusions: Overall, results revealed a possible hepatic adaptive mechanism to counteract diet-induced steatosis in animal and hypercholesterolemia progression in humans. In particular, low serum lyso-PE18:2 suggests a suitable susceptibility/risk biomarker of HC in humans.

Thematic Areas: Saúde coletiva; Química; Odontología; Nutrition and dietetics; Nutrition & dietetics; Nutrição; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; General medicine; Farmacia; Engenharias iv; Enfermagem; Educação física; Critical care and intensive care medicine; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciência de alimentos; Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0261-5614

Author's mail: josepm.delbas@urv.cat; anna.pedret@urv.cat; elisabet.llaurado@urv.cat; manuel.suarez@urv.cat; rosa.sola@urv.cat; lluis.arola@urv.cat

Record's date: 2025-02-18

Paper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.clinicalnutritionjournal.com/article/S0261-5614(21)00543-4/fulltext

Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/

Paper original source: Clinical Nutrition. 41 (2): 489-499

APA: Calderon-Perez, Lorena; Suarez-Garcia, Susana; Pedret, Anna; Suarez, Manuel; Llaurado, Elisabet; Rubio, Laura; del Bas, Josep M; Caimari, Antoni; Puig (2022). Serum lysophospholipidome of dietary origin as a suitable susceptibility/risk biomarker of human hypercholesterolemia: A cross-sectional study. Clinical Nutrition, 41(2), 489-499. DOI: 10.1016/j.clnu.2021.11.033

Article's DOI: 10.1016/j.clnu.2021.11.033

Entity: Universitat Rovira i Virgili

Journal publication year: 2022

Publication Type: Journal Publications