Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

SALDI-MS and SERS Multimodal Imaging: One Nanostructured Substrate to Rule Them Both

  • Identification data

    Identifier: imarina:9245547
    Authors:
    Iakab SABaquer GLafuente MPina MPRamírez JLRàfols PCorreig-Blanchar XGarcía-Altares M
    Abstract:
    Imaging techniques based on mass spectrometry or spectroscopy methods inform in situ about the chemical composition of biological tissues or organisms, but they are sometimes limited by their specificity, sensitivity, or spatial resolution. Multimodal imaging addresses these limitations by combining several imaging modalities; however, measuring the same sample with the same preparation using multiple imaging techniques is still uncommon due to the incompatibility between substrates, sample preparation protocols, and data formats. We present a multimodal imaging approach that employs a gold-coated nanostructured silicon substrate to couple surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and surface-enhanced Raman spectroscopy (SERS). Our approach integrates both imaging modalities by using the same substrate, sample preparation, and data analysis software on the same sample, allowing the coregistration of both images. We transferred molecules from clean fingertips and fingertips covered with plasticine modeling clay onto our nanostructure and analyzed their chemical composition and distribution by SALDI-MS and SERS. Multimodal analysis located the traces of plasticine on fingermarks and provided chemical information on the composition of the clay. Our multimodal approach effectively combines the advantages of mass spectrometry and vibrational spectroscopy with the signal enhancing abilities of our nanostructured substrate.
  • Others:

    Author, as appears in the article.: Iakab SA; Baquer G; Lafuente M; Pina MP; Ramírez JL; Ràfols P; Correig-Blanchar X; García-Altares M
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: Baquer Gómez, Gerard Sergi / Correig Blanchar, Francesc Xavier / Garcia-Altares Pérez, Maria / Ràfols Soler, Pere / Ramírez Falo, José Luis
    Keywords: Desorption/ionization mass-spectrometry workflow vibrational spectroscopy scattering raman-spectroscopy maldi-tof images database breast-cancer
    Abstract: Imaging techniques based on mass spectrometry or spectroscopy methods inform in situ about the chemical composition of biological tissues or organisms, but they are sometimes limited by their specificity, sensitivity, or spatial resolution. Multimodal imaging addresses these limitations by combining several imaging modalities; however, measuring the same sample with the same preparation using multiple imaging techniques is still uncommon due to the incompatibility between substrates, sample preparation protocols, and data formats. We present a multimodal imaging approach that employs a gold-coated nanostructured silicon substrate to couple surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and surface-enhanced Raman spectroscopy (SERS). Our approach integrates both imaging modalities by using the same substrate, sample preparation, and data analysis software on the same sample, allowing the coregistration of both images. We transferred molecules from clean fingertips and fingertips covered with plasticine modeling clay onto our nanostructure and analyzed their chemical composition and distribution by SALDI-MS and SERS. Multimodal analysis located the traces of plasticine on fingermarks and provided chemical information on the composition of the clay. Our multimodal approach effectively combines the advantages of mass spectrometry and vibrational spectroscopy with the signal enhancing abilities of our nanostructured substrate.
    Thematic Areas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: maria.garcia-altares@urv.cat pere.rafols@urv.cat gerard.baquer@estudiants.urv.cat gerard.baquer@estudiants.urv.cat joseluis.ramirez@urv.cat xavier.correig@urv.cat
    Author identifier: 0000-0002-9240-4058 0000-0002-4433-4972 0000-0002-4433-4972 0000-0001-8231-4019 0000-0002-6902-3054
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://pubs.acs.org/doi/10.1021/acs.analchem.1c04118
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Analytical Chemistry. 94 (6): 2785-2793
    APA: Iakab SA; Baquer G; Lafuente M; Pina MP; Ramírez JL; Ràfols P; Correig-Blanchar X; García-Altares M (2022). SALDI-MS and SERS Multimodal Imaging: One Nanostructured Substrate to Rule Them Both. Analytical Chemistry, 94(6), 2785-2793. DOI: 10.1021/acs.analchem.1c04118
    Article's DOI: 10.1021/acs.analchem.1c04118
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Chemistry, Analytical
    Desorption/ionization mass-spectrometry
    workflow
    vibrational spectroscopy
    scattering
    raman-spectroscopy
    maldi-tof
    images
    database
    breast-cancer
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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