Articles producció científica> Bioquímica i Biotecnologia

Time-of-Day Circadian Modulation of Grape-Seed Procyanidin Extract (GSPE) in Hepatic Mitochondrial Dynamics in Cafeteria-Diet-Induced Obese Rats

  • Identification data

    Identifier: imarina:9245550
    Authors:
    Rodríguez RMCortés-Espinar AJSoliz-Rueda JRFeillet-Coudray CCasas FColom-Pellicer MAragonès GAvila-Román JMuguerza BMulero MSalvadó MJ
    Abstract:
    Major susceptibility to alterations in liver function (e.g., hepatic steatosis) in a prone environment due to circadian misalignments represents a common consequence of recent sociobiological behavior (i.e., food excess and sleep deprivation). Natural compounds and, more concisely, polyphe-nols have been shown as an interesting tool for fighting against metabolic syndrome and related consequences. Furthermore, mitochondria have been identified as an important target for mediation of the health effects of these compounds. Additionally, mitochondrial function and dynamics are strongly regulated in a circadian way. Thus, we wondered whether some of the beneficial effects of grape-seed procyanidin extract (GSPE) on metabolic syndrome could be mediated by a circadian modulation of mitochondrial homeostasis. For this purpose, rats were subjected to “standard”, “cafeteria” and “cafeteria diet + GSPE” treatments (n = 4/group) for 9 weeks (the last 4 weeks, GSPE/vehicle) of treatment, administering the extract/vehicle at diurnal or nocturnal times (ZT0 or ZT12). For circadian assessment, one hour after turning the light on (ZT1), animals were sacrificed every 6 h (ZT1, ZT7, ZT13 and ZT19). Interestingly, GSPE was able to restore the rhythm on clock hepatic genes (Bmal1, Per2, Cry1, Rorα), as this correction was more evident in nocturnal treatment. Additionally, during nocturnal treatment, an increase in hepatic fusion genes and a decrease in fission genes were observed. Regarding mitochondrial complex activity, there was a strong effect of cafeteria diet at nearly all ZTs, and GSPE was able to restore activity at discrete ZTs, mainly in the diurnal treatment (ZT0). Furthermore, a differential behavior was observed in tricarboxylic acid (TCA) metabolites between GSPE diurnal and
  • Others:

    Author, as appears in the article.: Rodríguez RM; Cortés-Espinar AJ; Soliz-Rueda JR; Feillet-Coudray C; Casas F; Colom-Pellicer M; Aragonès G; Avila-Román J; Muguerza B; Mulero M; Salvadó MJ
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Aragonès Bargalló, Gerard / Colom Pellicer, Marina / Cortés Espinar, Antonio Jesús / Muguerza Marquínez, Maria Begoña / Mulero Abellán, Miguel / Rodriguez, Romina Mariel / Salvadó Rovira, Maria Josepa / Soliz Rueda, Jorge Ricardo
    Keywords: Zeitgebers Obesity Nutrition Mitochondrial dynamics Hepatic metabolism Grape-seed procyanidin extract Fatty liver-disease Clock genes Circadian rhythms zeitgebers supplementation resveratrol respiratory-chain polyphenols overexpression obesity nutrition mitochondrial dynamics metabolic syndrome hepatic metabolism fission dysfunction clock genes clock circadian rhythms
    Abstract: Major susceptibility to alterations in liver function (e.g., hepatic steatosis) in a prone environment due to circadian misalignments represents a common consequence of recent sociobiological behavior (i.e., food excess and sleep deprivation). Natural compounds and, more concisely, polyphe-nols have been shown as an interesting tool for fighting against metabolic syndrome and related consequences. Furthermore, mitochondria have been identified as an important target for mediation of the health effects of these compounds. Additionally, mitochondrial function and dynamics are strongly regulated in a circadian way. Thus, we wondered whether some of the beneficial effects of grape-seed procyanidin extract (GSPE) on metabolic syndrome could be mediated by a circadian modulation of mitochondrial homeostasis. For this purpose, rats were subjected to “standard”, “cafeteria” and “cafeteria diet + GSPE” treatments (n = 4/group) for 9 weeks (the last 4 weeks, GSPE/vehicle) of treatment, administering the extract/vehicle at diurnal or nocturnal times (ZT0 or ZT12). For circadian assessment, one hour after turning the light on (ZT1), animals were sacrificed every 6 h (ZT1, ZT7, ZT13 and ZT19). Interestingly, GSPE was able to restore the rhythm on clock hepatic genes (Bmal1, Per2, Cry1, Rorα), as this correction was more evident in nocturnal treatment. Additionally, during nocturnal treatment, an increase in hepatic fusion genes and a decrease in fission genes were observed. Regarding mitochondrial complex activity, there was a strong effect of cafeteria diet at nearly all ZTs, and GSPE was able to restore activity at discrete ZTs, mainly in the diurnal treatment (ZT0). Furthermore, a differential behavior was observed in tricarboxylic acid (TCA) metabolites between GSPE diurnal and nocturnal administration times. Therefore, GSPE may serve as a nutritional preventive strategy in the recovery of hepatic-related metabolic disease by modulating mitochondrial dynamics, which is concomitant to the restoration of the hepatic circadian machinery.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: marina.colom@urv.cat antoniojesus.cortes@urv.cat marina.colom@urv.cat marina.colom@urv.cat jorgericardo.soliz@urv.cat rominamariel.rodriguez@urv.cat rominamariel.rodriguez@urv.cat antoniojesus.cortes@urv.cat antoniojesus.cortes@urv.cat miquel.mulero@urv.cat gerard.aragones@urv.cat begona.muguerza@urv.cat
    Author identifier: 0000-0001-7384-8588
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2072-6643/14/4/774
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Nutrients. 14 (4):
    APA: Rodríguez RM; Cortés-Espinar AJ; Soliz-Rueda JR; Feillet-Coudray C; Casas F; Colom-Pellicer M; Aragonès G; Avila-Román J; Muguerza B; Mulero M; Salvad (2022). Time-of-Day Circadian Modulation of Grape-Seed Procyanidin Extract (GSPE) in Hepatic Mitochondrial Dynamics in Cafeteria-Diet-Induced Obese Rats. Nutrients, 14(4), -. DOI: 10.3390/nu14040774
    Article's DOI: 10.3390/nu14040774
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Food Science,Nutrition & Dietetics,Nutrition and Dietetics
    Zeitgebers
    Obesity
    Nutrition
    Mitochondrial dynamics
    Hepatic metabolism
    Grape-seed procyanidin extract
    Fatty liver-disease
    Clock genes
    Circadian rhythms
    zeitgebers
    supplementation
    resveratrol
    respiratory-chain
    polyphenols
    overexpression
    obesity
    nutrition
    mitochondrial dynamics
    metabolic syndrome
    hepatic metabolism
    fission
    dysfunction
    clock genes
    clock
    circadian rhythms
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Psicología
    Planejamento urbano e regional / demografia
    Nutrition and dietetics
    Nutrition & dietetics
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science
    Farmacia
    Engenharias iv
    Engenharias ii
    Enfermagem
    Educação física
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
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