Author, as appears in the article.: Rodriguez, Romina M; Cortes-Espinar, Antonio J; Soliz-Rueda, Jorge R; Feillet-Coudray, Christine; Casas, Francois; Colom-Pellicer, Marina; Aragones, Gerard; Avila-Roman, Javier; Muguerza, Begona; Mulero, Miquel; Salvado, Maria Josepa
Department: Bioquímica i Biotecnologia
URV's Author/s: Aragonès Bargalló, Gerard / Ávila Román, Francisco Javier / Colom Pellicer, Marina / Cortés Espinar, Antonio Jesús / Muguerza Marquínez, Maria Begoña / Mulero Abellán, Miguel / Rodriguez, Romina Mariel / Salvadó Rovira, Maria Josepa / Soliz Rueda, Jorge Ricardo
Keywords: Zeitgebers; Vitis; Rats, wistar; Rats; Proanthocyanidins; Obesity; Nutrition; Mitochondrial dynamics; Liver; Hepatic metabolism; Grape-seed procyanidin extract; Grape seed extract; Fatty liver-disease; Diet; Clock genes; Circadian rhythms; Animals; zeitgebers; supplementation; resveratrol; respiratory-chain; polyphenols; overexpression; obesity; nutrition; mitochondrial dynamics; metabolic syndrome; hepatic metabolism; fission; dysfunction; clock genes; clock; circadian rhythms
Abstract: Major susceptibility to alterations in liver function (e.g., hepatic steatosis) in a prone environment due to circadian misalignments represents a common consequence of recent sociobiological behavior (i.e., food excess and sleep deprivation). Natural compounds and, more concisely, polyphe-nols have been shown as an interesting tool for fighting against metabolic syndrome and related consequences. Furthermore, mitochondria have been identified as an important target for mediation of the health effects of these compounds. Additionally, mitochondrial function and dynamics are strongly regulated in a circadian way. Thus, we wondered whether some of the beneficial effects of grape-seed procyanidin extract (GSPE) on metabolic syndrome could be mediated by a circadian modulation of mitochondrial homeostasis. For this purpose, rats were subjected to “standard”, “cafeteria” and “cafeteria diet + GSPE” treatments (n = 4/group) for 9 weeks (the last 4 weeks, GSPE/vehicle) of treatment, administering the extract/vehicle at diurnal or nocturnal times (ZT0 or ZT12). For circadian assessment, one hour after turning the light on (ZT1), animals were sacrificed every 6 h (ZT1, ZT7, ZT13 and ZT19). Interestingly, GSPE was able to restore the rhythm on clock hepatic genes (Bmal1, Per2, Cry1, Rorα), as this correction was more evident in nocturnal treatment. Additionally, during nocturnal treatment, an increase in hepatic fusion genes and a decrease in fission genes were observed. Regarding mitochondrial complex activity, there was a strong effect of cafeteria diet at nearly all ZTs, and GSPE was able to restore activity at discrete ZTs, mainly in the diurnal treatment (ZT0). Furthermore, a differential behavior was observed in tricarboxylic acid (TCA) metabolites between GSPE diurnal and nocturnal administration times. Therefore, GSPE may serve as a nutritional preventive strategy in the recovery of hepatic-related metabolic disease by modulating mitochondrial dynamics, which is concomitant to the restoration of the hepatic circadian machinery.
Thematic Areas: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Psicología; Planejamento urbano e regional / demografia; Nutrition and dietetics; Nutrition & dietetics; Nutrição; Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Food science; Farmacia; Engenharias iv; Engenharias ii; Enfermagem; Educação física; Economia; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências agrárias i; Ciência de alimentos; Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: antoniojesus.cortes@urv.cat; franciscojavier.avila@urv.cat; marina.colom@urv.cat; marina.colom@urv.cat; jorgericardo.soliz@urv.cat; rominamariel.rodriguez@urv.cat; rominamariel.rodriguez@urv.cat; antoniojesus.cortes@urv.cat; antoniojesus.cortes@urv.cat; miquel.mulero@urv.cat; gerard.aragones@urv.cat; begona.muguerza@urv.cat
Record's date: 2025-02-08
Paper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2072-6643/14/4/774
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Nutrients. 14 (4): 774-
APA: Rodriguez, Romina M; Cortes-Espinar, Antonio J; Soliz-Rueda, Jorge R; Feillet-Coudray, Christine; Casas, Francois; Colom-Pellicer, Marina; Aragones, G (2022). Time-of-Day Circadian Modulation of Grape-Seed Procyanidin Extract (GSPE) in Hepatic Mitochondrial Dynamics in Cafeteria-Diet-Induced Obese Rats. Nutrients, 14(4), 774-. DOI: 10.3390/nu14040774
Article's DOI: 10.3390/nu14040774
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV