Imbalances in TCA, Short Fatty Acids and One‐Carbon Metabolisms as Important Features of Homeostatic Disruption Evidenced by a Multi‐Omics Integrative Approach of LPS‐Induced Chronic Inflammation in Male Wistar Rats

Identifier:  imarina:9246566

Handle: https://hdl.handle.net/20.500.11797/imarina9246566

Authors:  Hernandez-Baixauli, J; Abasolo, N; Palacios-Jordan, H; Foguet-Romero, E; Suñol, D; Galofré, M; Caimari, A; Baselga-Escudero, L; Del Bas, JM; Mulero, M

Abstract:
Chronic inflammation is an important risk factor in a broad variety of physical and mental disorders leading to highly prevalent non‐communicable diseases (NCDs). However, there is a need for a deeper understanding of this condition and its progression to the disease state. For this reason, it is important to define metabolic pathways and complementary biomarkers associated with homeostatic disruption in chronic inflammation. To achieve that, male Wistar rats were subjected to intraperitoneal and intermittent injections with saline solution or increasing lipopolysaccharide (LPS) concentrations (0.5, 5 and 7.5 mg/kg) thrice a week for 31 days. Biochemical and inflammatory parameters were measured at the end of the study. To assess the omics profile, GC‐qTOF and UHPLC‐qTOF were performed to evaluate plasma metabolome;1H‐NMR was used to evaluate urine metabolome; additionally, shotgun metagenomics sequencing was carried out to characterize the cecum microbiome. The chronicity of inflammation in the study was evaluated by the monitoring of monocyte chemoattractant protein‐1 (MCP‐1) during the different weeks of the experimental process. At the end of the study, together with the increased levels of MCP‐1, levels of interleukin‐ 6 (IL‐6), tumour necrosis factor alpha (TNF‐α) and prostaglandin E2 (PGE2) along with 8‐isopros-tanes (an indicative of oxidative stress) were significantly increased (p‐value < 0.05). The leading features implicated in the current model were tricarboxylic acid (TCA) cycle intermediates (i.e., alpha‐ketoglutarate, aconitic acid, malic acid, fumaric acid and succinic acid); lipids such as specific cholesterol esters (ChoEs), lysophospholipids (LPCs) and phosphatidylcholines (PCs); and glycine, as well as N, N‐dimethylglycine, which are related to one‐carbon (1C) metabolism. These metabolites point towards mitochondrial metabolism through TCA cycle, β‐oxidation of fatty acids and 1C metabolism as interconnected pathways that could reveal the metabolic effects of chronic inflammation induced by LPS administration. These results provide deeper knowledge concerning the impact of chronic inflammation on the disruption of metabolic homeostasis.