Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis

  • Identification data

    Identifier: imarina:9249038
    Authors:
    Vitenberga-Verza ZPilmane MŠerstnova KMelderis IGontar ŁKochański MDrutowska AMaróti GPrieto-Simón B
    Abstract:
    In naturally occurring bovine mastitis, effects of infection depend on the host inflammatory response, including the effects of secreted cytokines. Knowledge about the inflammatory and regulatory cytokines in milk cells of free-stall barn dairy cows and in naturally occurring mastitis is lacking as most studies focus on induced mastitis. Hereby, the aim of the study was to determine inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis. The following examinations of milk samples were performed: differential counting of somatic cells (SCC), bacteriological examination, and immunocytochemical analysis. Mean SCC increased in subclinical and clinical mastitis cases. The number of pathogenic mastitis-causing bacteria on plates increased in subclinical mastitis cases but decreased in clinical mastitis. The inflammatory and regulatory markers in the milk cells of healthy cows showed the highest mean cell numbers (%). In mastitis cases, immunoreactivity was more pronounced for IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-α, and IFN-γ. Data about subclinical and clinical mastitis demonstrate inflammatory responses to intramammary infection driven by IL-1α, IL-4, and IL-17A. Moreover, the host defense response in mastitis is characterized by continuation or resolution of initial inflammation. IL-12 and INF-γ immunoreactivity was recognized to differ mastitis cases from the relative health status.
  • Others:

    Author, as appears in the article.: Vitenberga-Verza Z; Pilmane M; Šerstnova K; Melderis I; Gontar Ł; Kochański M; Drutowska A; Maróti G; Prieto-Simón B
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: Prieto Simón, Beatriz
    Keywords: Milk Mastitis Inflammation Immunohistochemistry Diagnosis Cytokines Bovine mammary-gland Bovine staphylococcus-aureus somatic-cells oxide affect secretion milk intramammary infection interferon-gamma innate immune-response inflammation immunohistochemistry etiologic agents escherichia-coli epithelial-cells diagnosis cytokines bovine
    Abstract: In naturally occurring bovine mastitis, effects of infection depend on the host inflammatory response, including the effects of secreted cytokines. Knowledge about the inflammatory and regulatory cytokines in milk cells of free-stall barn dairy cows and in naturally occurring mastitis is lacking as most studies focus on induced mastitis. Hereby, the aim of the study was to determine inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis. The following examinations of milk samples were performed: differential counting of somatic cells (SCC), bacteriological examination, and immunocytochemical analysis. Mean SCC increased in subclinical and clinical mastitis cases. The number of pathogenic mastitis-causing bacteria on plates increased in subclinical mastitis cases but decreased in clinical mastitis. The inflammatory and regulatory markers in the milk cells of healthy cows showed the highest mean cell numbers (%). In mastitis cases, immunoreactivity was more pronounced for IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-α, and IFN-γ. Data about subclinical and clinical mastitis demonstrate inflammatory responses to intramammary infection driven by IL-1α, IL-4, and IL-17A. Moreover, the host defense response in mastitis is characterized by continuation or resolution of initial inflammation. IL-12 and INF-γ immunoreactivity was recognized to differ mastitis cases from the relative health status.
    Thematic Areas: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: beatriz.prieto-simon@urv.cat
    Author identifier: 0000-0001-8016-1565
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2076-0817/11/3/372
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Pathogens. 11 (3):
    APA: Vitenberga-Verza Z; Pilmane M; Šerstnova K; Melderis I; Gontar Ł; Kochański M; Drutowska A; Maróti G; Prieto-Simón B (2022). Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis. Pathogens, 11(3), -. DOI: 10.3390/pathogens11030372
    Article's DOI: 10.3390/pathogens11030372
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Immunology and Allergy,Immunology and Microbiology (Miscellaneous),Infectious Diseases,Microbiology,Microbiology (Medical),Molecular Biology
    Milk
    Mastitis
    Inflammation
    Immunohistochemistry
    Diagnosis
    Cytokines
    Bovine mammary-gland
    Bovine
    staphylococcus-aureus
    somatic-cells
    oxide affect secretion
    milk
    intramammary infection
    interferon-gamma
    innate immune-response
    inflammation
    immunohistochemistry
    etiologic agents
    escherichia-coli
    epithelial-cells
    diagnosis
    cytokines
    bovine
    Molecular biology
    Microbiology (medical)
    Microbiology
    Infectious diseases
    Immunology and microbiology (miscellaneous)
    Immunology and microbiology (all)
    Immunology and allergy
    General immunology and microbiology
    Farmacia
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