Author, as appears in the article.: Vitenberga-Verza Z; Pilmane M; Šerstnova K; Melderis I; Gontar Ł; Kochański M; Drutowska A; Maróti G; Prieto-Simón B
Department: Enginyeria Electrònica, Elèctrica i Automàtica
URV's Author/s: Prieto Simón, Beatriz
Keywords: Milk Mastitis Inflammation Immunohistochemistry Diagnosis Cytokines Bovine mammary-gland Bovine staphylococcus-aureus somatic-cells oxide affect secretion milk intramammary infection interferon-gamma innate immune-response inflammation immunohistochemistry etiologic agents escherichia-coli epithelial-cells diagnosis cytokines bovine
Abstract: In naturally occurring bovine mastitis, effects of infection depend on the host inflammatory response, including the effects of secreted cytokines. Knowledge about the inflammatory and regulatory cytokines in milk cells of free-stall barn dairy cows and in naturally occurring mastitis is lacking as most studies focus on induced mastitis. Hereby, the aim of the study was to determine inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis. The following examinations of milk samples were performed: differential counting of somatic cells (SCC), bacteriological examination, and immunocytochemical analysis. Mean SCC increased in subclinical and clinical mastitis cases. The number of pathogenic mastitis-causing bacteria on plates increased in subclinical mastitis cases but decreased in clinical mastitis. The inflammatory and regulatory markers in the milk cells of healthy cows showed the highest mean cell numbers (%). In mastitis cases, immunoreactivity was more pronounced for IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-α, and IFN-γ. Data about subclinical and clinical mastitis demonstrate inflammatory responses to intramammary infection driven by IL-1α, IL-4, and IL-17A. Moreover, the host defense response in mastitis is characterized by continuation or resolution of initial inflammation. IL-12 and INF-γ immunoreactivity was recognized to differ mastitis cases from the relative health status.
Thematic Areas: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: beatriz.prieto-simon@urv.cat
Author identifier: 0000-0001-8016-1565
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2076-0817/11/3/372
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Pathogens. 11 (3):
APA: Vitenberga-Verza Z; Pilmane M; Šerstnova K; Melderis I; Gontar Ł; Kochański M; Drutowska A; Maróti G; Prieto-Simón B (2022). Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis. Pathogens, 11(3), -. DOI: 10.3390/pathogens11030372
Article's DOI: 10.3390/pathogens11030372
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications