Author, as appears in the article.: Colom-Pellicer, Marina; Rodriguez, Romina M.; Navarro-Masip, Elia; Bravo, Francisca Isabel; Mulero, Miquel; Arola, Lluis; Aragones, Gerard;
Department: Bioquímica i Biotecnologia
URV's Author/s: Aragonès Bargalló, Gerard / Arola Ferrer, Luis Maria / Bravo Vázquez, Francisca Isabel / Colom Pellicer, Marina / Mulero Abellán, Miguel / Navarro Masip, Èlia / RODRÍGUEZ SÁNCHEZ, ROSA MARIA
Keywords: Transplantation Mice Healthy Fat Clocks
Abstract: Background Grape-seed proanthocyanidin extract (GSPE) improve white adipose tissue (WAT) expansion during diet-induced obesity. However, because adipose metabolism is synchronized by circadian rhythms, it is plausible to speculate that the bioactivity of dietary proanthocyanidins could be influenced by the time-of-day in which they are consumed. Therefore, the aim of the present study was to determine the interaction between zeitgeber time (ZT) and GSPE consumption on the functionality of WAT in rats with diet-induced obesity. Methods Male Wistar rats were fed a cafeteria diet for 9 weeks. After 5 weeks, the animals were supplemented with 25 mg GSPE/kg for 4 weeks at the beginning of the light/rest phase (ZT0) or of the dark/active phase (ZT12). Body fat content was determined by nuclear magnetic resonance and histological analyses were performed in the epididymal (EWAT) and inguinal (IWAT) fat depots to determine adipocyte size and number. In addition, the expression of genes related to adipose metabolism and circadian clock function were analyzed by qPCR. Results GSPE consumption at ZT0 was associated with a potential antidiabetic effect without affecting adiposity and energy intake and downregulating the gene expression of inflammatory markers in EWAT. In contrast, GSPE consumption at ZT12 improved adipose tissue expansion decreasing adipocyte size in IWAT. In accordance with this adipogenic activity, the expression of genes involved in fatty acid metabolism were downregulated at ZT12 in IWAT. In turn, GSPE consumption at ZT12, but not at ZT0, repressed the expression of the clock gene Cry1 in IWAT. Conclusions The interaction between ZT and GSPE consumption influenced the metabolic response of WAT in a tissue-specific manner. Understanding the impact of circadian clock on adipose metabolism and how this is regulated by polyphenols will provide new insights for the management of obesity.
Thematic Areas: Serviço social Saúde coletiva Psicología Nutrition and dietetics Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: franciscaisabel.bravo@urv.cat marina.colom@urv.cat lluis.arola@urv.cat gerard.aragones@urv.cat miquel.mulero@urv.cat elia.navarro@estudiants.urv.cat elia.navarro@estudiants.urv.cat marina.colom@urv.cat marina.colom@urv.cat
Author identifier: 0000-0002-6468-3088 0000-0003-2767-1974
Record's date: 2024-02-25
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.nature.com/articles/s41366-022-01132-0
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: International Journal Of Obesity. 46 (7): 1394-1402
APA: Colom-Pellicer, Marina; Rodriguez, Romina M.; Navarro-Masip, Elia; Bravo, Francisca Isabel; Mulero, Miquel; Arola, Lluis; Aragones, Gerard; (2022). Time-of-day dependent effect of proanthocyanidins on adipose tissue metabolism in rats with diet-induced obesity. International Journal Of Obesity, 46(7), 1394-1402. DOI: 10.1038/s41366-022-01132-0
Article's DOI: 10.1038/s41366-022-01132-0
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV