Author, as appears in the article.: Masip J; Rallón N; Yeregui E; Olona M; Resino S; Benito JM; Viladés C; García-Pardo G; Alcamí J; Ruiz-Mateos E; Gómez-Bertomeu F; Vargas M; Navarro M; Oteo JA; Pineda JA; Martí A; Alba V; Vidal F; Peraire J; Rull A
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Garcia Pardo, Graciano / Gomez Bertomeu, Frederic-Francesc / Martí Zaragoza, Àlex / Masip Morcillo, Jenifer / Olona Cabases, Maria Montserrat / Peraire Forner, José Joaquin / RULL AIXA, ANNA / Vidal Marsal, Francisco / Vilades Laborda, Consuelo Gloria / Yeregui Etxeberria, Elena
Keywords: Viral Metabolomics Mass spectrometry Long-term Lipidomics Kreb's cycle Hiv infection Elite controllers (ecs) Elite controllers viral profiles nonprogressors mass spectrometry long-term lipidomics kreb's cycle hiv infection glucose elite controllers (ecs) cycle activation absence
Abstract: Long-term elite controllers (LTECs) are a fascinating small subset of HIV individuals with viral and immunological HIV control in the long term that have been designated as models of an HIV functional cure. However, data on the LTEC phenotype are still scarce, and hence, the metabolomics and lipidomics signatures in the LTEC-extreme phenotype, LTECs with more than 10 years of viral and immunological HIV control, could be pivotal to finding the keys for functional HIV remission. Metabolomics and lipidomics analyses were performed using high-resolution mass spectrometry (ultra-high-performance liquid chromatography-electrospray ionization-quadrupole time of flight [UHPLC-(ESI) qTOF] in plasma samples of 13 patients defined as LTEC-extreme, a group of 20 LTECs that lost viral and/or immunological control during the follow-up study (LTEC-losing) and 9 EC patients with short-term viral and immunological control (less than 5 years; no-LTEC patients). Long-term viral and immunological HIV-1 control was found to be strongly associated with elevated tricarboxylic acid (TCA) cycle function. Interestingly, of the nine metabolites identified in the TCA cycle, α-ketoglutaric acid (p = 0.004), a metabolite implicated in the activation of the mTOR complex, a modulator of HIV latency and regulator of several biological processes, was found to be a key metabolite in the persistent control. On the other hand, a lipidomics panel combining 45 lipid species showed an optimal percentage of separation and an ability to differentiate LTEC-extreme from LTEC-losing, revealing that an elevated lipidomics plasma profile could be a predictive factor for the reignition of viral replication in LTEC individuals.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Immunology and allergy Immunology Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Biodiversidade
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: graciano.garcia@urv.cat frederic-francesc.gomez@urv.cat joaquim.peraire@urv.cat consuelo.vilades@urv.cat francesc.vidal@urv.cat jenifer.masip@estudiants.urv.cat jenifer.masip@estudiants.urv.cat alex.marti@estudiants.urv.cat elena.yeregui@estudiants.urv.cat
Author identifier: 0000-0002-8039-2889 0000-0001-7808-5479 0000-0002-2991-9593 0000-0002-6692-6186
Record's date: 2024-05-27
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.frontiersin.org/articles/10.3389/fimmu.2022.822272/full
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Frontiers In Immunology. 13 822272-
APA: Masip J; Rallón N; Yeregui E; Olona M; Resino S; Benito JM; Viladés C; García-Pardo G; Alcamí J; Ruiz-Mateos E; Gómez-Bertomeu F; Vargas M; Navarro M; (2022). Elevated α-Ketoglutaric Acid Concentrations and a Lipid-Balanced Signature Are the Key Factors in Long-Term HIV Control. Frontiers In Immunology, 13(), 822272-. DOI: 10.3389/fimmu.2022.822272
Article's DOI: 10.3389/fimmu.2022.822272
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV