Grape-Seed Procyanidin Extract (GSPE) Seasonal-Dependent Modulation of Glucose and Lipid Metabolism in the Liver of Healthy F344 Rats

Author, as appears in the article.: Rodriguez, Romina M; Colom-Pellicer, Marina; Blanco, Jordi; Calvo, Enrique; Aragones, Gerard; Mulero, Miquel

Department: Bioquímica i Biotecnologia; Ciències Mèdiques Bàsiques

URV's Author/s: Aragonès Bargalló, Gerard / Blanco Pérez, Jordi / Calvo Manso, Enrique / Colom Pellicer, Marina / Mulero Abellán, Miguel / Rodriguez, Romina Mariel

Keywords: Vitis; Seasons; Seasonal; Rats, inbred f344; Rats; Proanthocyanidins; Proanthocyanidin extract; Photoperiod; Oxidative stress; Melatonin; Mammals; Liver; Lipid metabolism; Gspe; Grape seed extract; Glycogen-phosphorylase; Glucose; Gene-expression; Er stress; Endoplasmic-reticulum stress; Density-lipoprotein cholesterol; Clock genes; Animals; Ampk; Activation

Abstract: Seasonality is gaining attention in the modulation of some physiological and metabolic functions in mammals. Furthermore, the consumption of natural compounds, such as GSPE, is steadily increasing. Consequently, in order to study the interaction of seasonal variations in day length over natural compounds' molecular effects, we carried out an animal study using photosensitive rats which were chronically exposed for 9 weeks to three photoperiods (L6, L18, and L12) in order to mimic the day length of different seasons (winter/summer/and autumn-spring). In parallel, animals were also treated either with GSPE 25 (mg/kg) or vehicle (VH) for 4 weeks. Interestingly, a seasonal-dependent GSPE modulation on the hepatic glucose and lipid metabolism was observed. For example, some metabolic genes from the liver (SREBP-1c, Gk, Acaca) changed their expression due to seasonality. Furthermore, the metabolomic results also indicated a seasonal influence on the GSPE effects associated with glucose-6-phosphate, D-glucose, and D-ribose, among others. These differential effects, which were also reflected in some plasmatic parameters (i.e., glucose and triglycerides) and hormones (corticosterone and melatonin), were also associated with significant changes in the expression of several hepatic circadian clock genes (Bmal1, Cry1, and Nr1d1) and ER stress genes (Atf6, Grp78, and Chop). Our results point out the importance of circannual rhythms in regulating metabolic homeostasis and suggest that seasonal variations (long or short photoperiods) affect hepatic metabolism in rats. Furthermore, they suggest that procyanidin consumption could be useful for the modulation of the photoperiod-dependent changes on glucose and lipid metabolism, whose alterations could be related to metabolic diseases (e.g., diabetes, obesity, and cardiovascular disease). Furthermore, even though the GSPE effect is not restricted to a specific photoperiod, our results suggest a more significant effect in the L18 condition.

Thematic Areas: Química; Molecular biology; Materiais; General medicine; Farmacia; Ensino; Biochemistry & molecular biology; Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: enrique.calvo@urv.cat; marina.colom@urv.cat; marina.colom@urv.cat; rominamariel.rodriguez@urv.cat; rominamariel.rodriguez@urv.cat; jordi.blanco@urv.cat; miquel.mulero@urv.cat; gerard.aragones@urv.cat

Record's date: 2025-02-08

Paper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2218-273X/12/6/839

Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/

Paper original source: Biomolecules. 12 (6): 839-

APA: Rodriguez, Romina M; Colom-Pellicer, Marina; Blanco, Jordi; Calvo, Enrique; Aragones, Gerard; Mulero, Miquel (2022). Grape-Seed Procyanidin Extract (GSPE) Seasonal-Dependent Modulation of Glucose and Lipid Metabolism in the Liver of Healthy F344 Rats. Biomolecules, 12(6), 839-. DOI: 10.3390/biom12060839

Article's DOI: 10.3390/biom12060839

Entity: Universitat Rovira i Virgili

Journal publication year: 2022

Publication Type: Journal Publications