Articles producció científica> Química Física i Inorgànica

Lethal Interactions of Atomically Precise Gold Nanoclusters and Pseudomonas aeruginosa and Staphylococcus aureus Bacterial Cells

  • Identification data

    Identifier: imarina:9267447
    Authors:
    Linklater DPLe Guével XBryant GBaulin VAPereiro EPerera PGTWandiyanto JVJuodkazis SIvanova EP
    Abstract:
    Ultrasmall metal nanoclusters (NCs) are employed in an array of diagnostic and therapeutic applications due to their tunable photoluminescence, high biocompatibility, polyvalent effect, ease of modification, and photothermal stability. However, gold nanoclusters' (AuNCs') intrinsically antimicrobial properties remain poorly explored and are not well understood. Here, we share an insight into the antimicrobial action of atomically precise AuNCs based on their ability to passively translocate across the bacterial membrane. Functionalized by a hydrophilic modified-bidentate sulfobetaine zwitterionic molecule (AuNC-ZwBuEt) or a more hydrophobic monodentate-thiolate, mercaptohexanoic acid (AuNC-MHA) molecule, 2 nm AuNCs were lethal to both Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. The bactericidal efficiency was found to be bacterial strain-, time-, and concentration-dependent. The direct visualizations of the translocation of AuNCs and AuNC-cell and subcellular interactions were investigated using cryo-soft X-ray nano-tomography, transmission electron microscopy (TEM), and scanning TEM energy-dispersive spectroscopy analyses. AuNC-MHA were identified in the bacterial cytoplasm within 30 min, without evidence of the loss of membrane integrity. It is proposed that the bactericidal effect of AuNCs is attributed to their size, which allows for efficient energy-independent translocation across the cell membrane. The internalization of both AuNCs caused massive internal damage to the cells, including collapsed subcellular structures and altered cell morphology, leading to the eventual loss of cellular integrity.
  • Others:

    Author, as appears in the article.: Linklater DP; Le Guével X; Bryant G; Baulin VA; Pereiro E; Perera PGT; Wandiyanto JV; Juodkazis S; Ivanova EP
    Department: Química Física i Inorgànica
    URV's Author/s: Baulin, Vladimir
    Keywords: Ultrasmall metal nanoclusters Translocation Nanotoxicity In-vitro Gold nanoclusters Antimicrobial effect translocation toxicity tomography size shape nanotoxicity nanoparticles morphology membranes gold nanoclusters antimicrobial effect
    Abstract: Ultrasmall metal nanoclusters (NCs) are employed in an array of diagnostic and therapeutic applications due to their tunable photoluminescence, high biocompatibility, polyvalent effect, ease of modification, and photothermal stability. However, gold nanoclusters' (AuNCs') intrinsically antimicrobial properties remain poorly explored and are not well understood. Here, we share an insight into the antimicrobial action of atomically precise AuNCs based on their ability to passively translocate across the bacterial membrane. Functionalized by a hydrophilic modified-bidentate sulfobetaine zwitterionic molecule (AuNC-ZwBuEt) or a more hydrophobic monodentate-thiolate, mercaptohexanoic acid (AuNC-MHA) molecule, 2 nm AuNCs were lethal to both Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. The bactericidal efficiency was found to be bacterial strain-, time-, and concentration-dependent. The direct visualizations of the translocation of AuNCs and AuNC-cell and subcellular interactions were investigated using cryo-soft X-ray nano-tomography, transmission electron microscopy (TEM), and scanning TEM energy-dispersive spectroscopy analyses. AuNC-MHA were identified in the bacterial cytoplasm within 30 min, without evidence of the loss of membrane integrity. It is proposed that the bactericidal effect of AuNCs is attributed to their size, which allows for efficient energy-independent translocation across the cell membrane. The internalization of both AuNCs caused massive internal damage to the cells, including collapsed subcellular structures and altered cell morphology, leading to the eventual loss of cellular integrity.
    Thematic Areas: Química Nanoscience and nanotechnology Nanoscience & nanotechnology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) Materiais Interdisciplinar General materials science Farmacia Engenharias iv Engenharias iii Engenharias ii Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Biodiversidade Astronomia / física Arquitetura, urbanismo e design
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: vladimir.baulin@urv.cat
    Author identifier: 0000-0003-2086-4271
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://pubs.acs.org/doi/10.1021/acsami.2c04410
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Acs Applied Materials & Interfaces. 14 (28): 32634-32645
    APA: Linklater DP; Le Guével X; Bryant G; Baulin VA; Pereiro E; Perera PGT; Wandiyanto JV; Juodkazis S; Ivanova EP (2022). Lethal Interactions of Atomically Precise Gold Nanoclusters and Pseudomonas aeruginosa and Staphylococcus aureus Bacterial Cells. Acs Applied Materials & Interfaces, 14(28), 32634-32645. DOI: 10.1021/acsami.2c04410
    Article's DOI: 10.1021/acsami.2c04410
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Medicine (Miscellaneous),Nanoscience & Nanotechnology,Nanoscience and Nanotechnology
    Ultrasmall metal nanoclusters
    Translocation
    Nanotoxicity
    In-vitro
    Gold nanoclusters
    Antimicrobial effect
    translocation
    toxicity
    tomography
    size
    shape
    nanotoxicity
    nanoparticles
    morphology
    membranes
    gold nanoclusters
    antimicrobial effect
    Química
    Nanoscience and nanotechnology
    Nanoscience & nanotechnology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    Materiais
    Interdisciplinar
    General materials science
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Biotecnología
    Biodiversidade
    Astronomia / física
    Arquitetura, urbanismo e design
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