Author, as appears in the article.: Alcazar, M.; Escribano, J.; Ferre, N.; Closa-Monasterolo, R.; Selma-Royo, M.; Feliu, A.; Castillejo, G.; Luque, V;Obemat2 0 Study Grp

Department: Medicina i Cirurgia

URV's Author/s: Closa Monasterolo, Ricardo / Escribano Subías, Joaquín / Ferre Pallas, Natalia / Luque Moreno, Verònica

Keywords: Overweight Microbiota Metabolically unhealthy obesity Intestinal microbiota Insulin sensitivity Feces Epidemiology Disease Childhood obesity Cardiometabolic risk Bacterial

Abstract: Background and aims: We aimed to describe and characterize the gut microbiota composition and diversity in children with obesity according to their metabolic health status. Methods: Anthropometry, Triglycerides, HDL cholesterol, HOMA-IR, and systolic and diastolic blood pressure (SBP, DBP) were evaluated (and z-score calculated) and faecal samples were collected from 191 children with obesity aged from 8 to 14. All children were classified depending on their cardiometabolic status in either a "metabolically healthy" (MHO; n = 106) or "metabolically unhealthy" (MUO; n = 85) group. Differences in gut microbiota taxonomies and diversity between groups (MUO vs MHO) were analysed. Alpha diversity index was calculated as Chao1 and Simpson's index, and b-diversity was calculated as Adonis Bray-Curtis index. Spearman's correlations and logistic regressions were performed to study the association between cardiometabolic health and the microbiota. Results: Children in the MUO presented significantly lower alpha diversity and richness than those in the MHO group (Chao1 index p = 0.021, Simpson's index p = 0.045, respectively), whereas microbiota bdiversity did not differ by the cardiometabolic health status (Adonis Bray-Curtis, R2 = 0.006; p = 0.155). The MUO group was characterized by lower relative abundances of the genera Christensenellaceae R7 group (MHO:1.42% [0.21-2.94]; MUO:0.47% [0.02-1.60], p < 0.004), and Akkermansia (MHO:0.26% [0.01 -2.19]; MUO:0.01% [0.00-0.36], p < 0.001) and higher relative abundances of Bacteroides (MHO:10.6% [4.64-18.5]; MUO:17.0% [7.18-27.4], p = 0.012) genus. After the adjustment by sex, age, and BMI, higher Akkermansia (OR: 0.86, CI: 0.75-0.97; p = 0.033), Christensenellaceae R7 group (OR: 0.86, 95% CI: 075 -0.98; p = 0.031) and Chao1 index (OR: 0.86, CI: 0.96-1.00; p = 0.023) represented a lower risk of the presence of one or more altered cardiovascular risk factors. Conclusion: Lower proportions of Christensenellaceae and Akkermansia and lower diversity and richness seem to be indicators of a metabolic unhealthy status in children with obesity. (c) 2022 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Thematic Areas: Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Educação física Critical care and intensive care medicine Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: veronica.luque@urv.cat joaquin.escribano@urv.cat natalia.ferre@urv.cat ricardo.closa@urv.cat

Author identifier: 0000-0003-2615-8175 0000-0002-5041-459X 0000-0002-2838-1525 0000-0002-9963-4163

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.clinicalnutritionjournal.com/article/S0261-5614(22)00192-3/fulltext

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Clinical Nutrition. 41 (8): 1680-1688

APA: Alcazar, M.; Escribano, J.; Ferre, N.; Closa-Monasterolo, R.; Selma-Royo, M.; Feliu, A.; Castillejo, G.; Luque, V;Obemat2 0 Study Grp (2022). Gut microbiota is associated with metabolic health in children with obesity. Clinical Nutrition, 41(8), 1680-1688. DOI: 10.1016/j.clnu.2022.06.007

Article's DOI: 10.1016/j.clnu.2022.06.007

Entity: Universitat Rovira i Virgili

Journal publication year: 2022

Publication Type: Journal Publications