Author, as appears in the article.: Segu, Helena; Jalsevac, Florijan; Pinent, Montserrat; Ardevol, Anna; Terra, Ximena; Teresa Blay, Maria;

Department: Bioquímica i Biotecnologia

URV's Author/s: Ardèvol Grau, Anna / Blay Olivé, Maria Teresa / JALSEVAC, FLORIJAN / Pinent Armengol, Montserrat / Segú Matamoros, Helena / Terra Barbadora, Ximena

Keywords: Wistar rats Western-style diet Weight Proanthocyanidins Intestine morphology Blind Absorptive surface area

Abstract: Western-style diet is an obesogenic diet for rodents and humans due to its content of saturated fat and refined sugars, mainly sucrose and, in consequence, sucrose-derived fructose. This type of diets relates with intestinal disturbances when consumed regularly. The aim of this work was to analyse the adaptive morphologic and functional changes at intestinal level derived from the unhealthy components of a Cafeteria diet in rats. The effect of grape seed proanthocyanidin extract (GSPE) in the prevention of diet-induced intestinal dysfunction was also analysed. Rats were fed a 17-week cafeteria diet (CAF) without or with oral-GSPE supplementation, either intermittent GSPE administration (SIT-CAF); last 10-day GSPE supplementation at doses of 100 mg/kg and 500 mg/kg day (CORR-100) and (CORR-500) or pre-supplementation with 500 mg/kg GSPE (PRE-CAF). GSPE-CAF supplemented groups showed similar results to CAF diet group regarding morphology and inflammatory score in the duodenum. As an adaptive response to diet, CAF increased intestinal absorptive surface (1.24-fold) all along the intestinal tract and specifically in the small intestine, duodenum, due to increase villus height and a higher villus/crypt ratio, in addition to increase in Goblet cell percentage and inflammatory index. Animals fed GSPE at the current doses and times had higher villus heights and absorptive surface similar to Cafeteria diet group. In the duodenum, villus height correlated with body weight at 17 week and negatively with MLCK gene expression. In the colon, villus height correlated with the percentage of goblet cells. In conclusion, the CAF diet produced adaptive modifications of the intestine by increasing the absorptive area of the small intestine, the percentage of goblet cells and the inflammatory index at the duodenal level. GSPE supplementation can partially reverse the intestinal morphological changes induced by the high fat/sucrose diet when administered intermittently.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: ximena.terra@urv.cat helena.segu@urv.cat florijan.jalsevac@urv.cat montserrat.pinent@urv.cat mteresa.blay@urv.cat anna.ardevol@urv.cat helena.segu@urv.cat

Author identifier: 0000-0003-1043-5844 0000-0003-3550-5378 0000-0002-6256-9847 0000-0003-0156-7538

Record's date: 2023-05-14

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2072-6643/14/13/2608

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Nutrients. 14 (13):

APA: Segu, Helena; Jalsevac, Florijan; Pinent, Montserrat; Ardevol, Anna; Terra, Ximena; Teresa Blay, Maria; (2022). Intestinal Morphometric Changes Induced by a Western-Style Diet in Wistar Rats and GSPE Counter-Regulatory Effect. Nutrients, 14(13), -. DOI: 10.3390/nu14132608

Article's DOI: 10.3390/nu14132608

Entity: Universitat Rovira i Virgili

Journal publication year: 2022

Publication Type: Journal Publications