Author, as appears in the article.: Pascual-Caro, Sergi; Borrull, Francesc; Calull, Marta; Aguilar, Carme;
Department: Química Analítica i Química Orgànica
URV's Author/s: Aguilar Anguera, Maria del Carmen / Borrull Ballarín, Francesc / Calull Blanch, Marta / Pascual Caro, Sergi
Keywords: Toxicological analysis Spe Sample preparation techniques Pipette tip solid-phase extraction Metabolites Lc-ms/ms Human urine Drugs of abuse Amphetamine
Abstract: Pipette tip solid-phase extraction facilitates the handling of low-volume samples and organic solvents in order to achieve more environmentally friendly pre-treatment sample techniques. The use of pipette tip extraction was examined for the quick and simple determination of a heterogeneous group of 40 drugs of abuse and some of their metabolites in urine by liquid chromatography coupled to tandem mass spectrometry. Several parameters were studied and optimized, including those which can affect extraction efficiencies, such as the amount of sorbent and the volumes and number of aspirating/dispensing cycles of the sample and organic solvents. The linear range of this method was between the quantification limit and 75 or 100 ng mL(-1). Detection limits between 0.025 and 0.500 ng mL(-1) and quantification limits from 0.100 to 1.500 ng mL(-1) were achieved, which are adequate to determine the studied compounds in urine from drug users. Finally, in order to prove its suitability in toxicological and forensic analyses, the method was successfully applied to 22 urine specimens from women who were starting a detoxification program. Cocaine was the most frequently detected substance, as its presence or the presence of its main metabolite was found in 86% of the analyzed samples.
Thematic Areas: Filtration and separation Chemistry, analytical Analytical chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: sergi.pascual@estudiants.urv.cat carme.aguilar@urv.cat marta.calull@urv.cat francesc.borrull@urv.cat
Author identifier: 0000-0001-8890-5100 0000-0002-5913-2599 0000-0002-5258-1949 0000-0003-2718-9336
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2297-8739/9/9/233
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Separations. 9 (9):
APA: Pascual-Caro, Sergi; Borrull, Francesc; Calull, Marta; Aguilar, Carme; (2022). Homemade Pipette Tip Solid-Phase Extraction for the Simultaneous Determination of 40 Drugs of Abuse in Urine by Liquid Chromatography-Tandem Mass Spectrometry. Separations, 9(9), -. DOI: 10.3390/separations9090233
Article's DOI: 10.3390/separations9090233
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV