Author, as appears in the article.: Manocchio F; Soliz-Rueda JR; Ribas-Latre A; Bravo FI; Arola-Arnal A; Suarez M; Muguerza B
Department: Bioquímica i Biotecnologia
URV's Author/s: Alkhoury, Nadine / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Manocchio, Francesca / Muguerza Marquínez, Maria Begoña / Ribas Latre, Aleix / Suárez Recio, Manuel
Keywords: Polyphenols Peripheral molecular clock Microrna Dose-dependent manner Cosinor-based rhythmometry Circadian rhythm rhythm rats polyphenols peripheral molecular clock mir-33a mir-122 microrna metabolism healthy expression cosinor-based rhythmometry bmal1 association
Abstract: Circadian rhythm is an endogenous and self-sustained timing system, responsible for the coordination of daily processes in 24-h timescale. It is regulated by an endogenous molecular clock, which is sensitive to external cues as light and food. We have previously shown that grape seed proanthocyanidins extract (GSPE) regulates the hepatic molecular clock. Moreover, GSPE is known to interact with some microRNAs (miRNAs). Therefore, the aim of this study was to evaluate if the activity of GSPE as modulator of hepatic clock genes could be mediated by miRNAs.250 mg/kg of GSPE was administered to Wistar rats before a 6-hour jet lag and sacrificed at different time points. GSPE modulated both expression of Bmal1 and miR-27b-3p in the liver. Cosinor-based analysis revealed that both Bmal1 and miR-27b-3p expression followed a circadian rhythm, a negative interaction between them, and the role of GSPE adjusting the hepatic peripheral clock via miRNA. Additionally, in vitro studies showed that Bmal1 was sensitive to GSPE (25 mg/L). However, this effect was independent of miR-27b-3p.miRNA regulation of peripheral clocks via GSPE may be part of a complex mechanism that involves the crosstalk with the central system rather than a direct effect. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
Thematic Areas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: franciscaisabel.bravo@urv.cat aleix.ribas@urv.cat begona.muguerza@urv.cat anna.arola@urv.cat manuel.suarez@urv.cat francesca.manocchio@estudiants.urv.cat francesca.manocchio@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat
Author identifier: 0000-0002-6468-3088 0000-0001-7384-8588 0000-0001-6529-1345 0000-0003-0122-8253
Record's date: 2024-02-25
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/full/10.1002/mnfr.202200443
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Molecular Nutrition & Food Research. 66 (23): e2200443-e2200443
APA: Manocchio F; Soliz-Rueda JR; Ribas-Latre A; Bravo FI; Arola-Arnal A; Suarez M; Muguerza B (2022). Grape Seed Proanthocyanidins Modulate the Hepatic Molecular Clock via MicroRNAs. Molecular Nutrition & Food Research, 66(23), e2200443-e2200443. DOI: 10.1002/mnfr.202200443
Article's DOI: 10.1002/mnfr.202200443
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV