Articles producció científica> Bioquímica i Biotecnologia

Grape Seed Proanthocyanidins Modulate the Hepatic Molecular Clock via MicroRNAs

  • Identification data

    Identifier: imarina:9282481
    Authors:
    Manocchio FSoliz-Rueda JRRibas-Latre ABravo FIArola-Arnal ASuarez MMuguerza B
    Abstract:
    Circadian rhythm is an endogenous and self-sustained timing system, responsible for the coordination of daily processes in 24-h timescale. It is regulated by an endogenous molecular clock, which is sensitive to external cues as light and food. We have previously shown that grape seed proanthocyanidins extract (GSPE) regulates the hepatic molecular clock. Moreover, GSPE is known to interact with some microRNAs (miRNAs). Therefore, the aim of this study was to evaluate if the activity of GSPE as modulator of hepatic clock genes could be mediated by miRNAs.250 mg/kg of GSPE was administered to Wistar rats before a 6-hour jet lag and sacrificed at different time points. GSPE modulated both expression of Bmal1 and miR-27b-3p in the liver. Cosinor-based analysis revealed that both Bmal1 and miR-27b-3p expression followed a circadian rhythm, a negative interaction between them, and the role of GSPE adjusting the hepatic peripheral clock via miRNA. Additionally, in vitro studies showed that Bmal1 was sensitive to GSPE (25 mg/L). However, this effect was independent of miR-27b-3p.miRNA regulation of peripheral clocks via GSPE may be part of a complex mechanism that involves the crosstalk with the central system rather than a direct effect. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
  • Others:

    Author, as appears in the article.: Manocchio F; Soliz-Rueda JR; Ribas-Latre A; Bravo FI; Arola-Arnal A; Suarez M; Muguerza B
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Alkhoury, Nadine / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Manocchio, Francesca / Muguerza Marquínez, Maria Begoña / Ribas Latre, Aleix / Suárez Recio, Manuel
    Keywords: Polyphenols Peripheral molecular clock Microrna Dose-dependent manner Cosinor-based rhythmometry Circadian rhythm rhythm rats polyphenols peripheral molecular clock mir-33a mir-122 microrna metabolism healthy expression cosinor-based rhythmometry bmal1 association
    Abstract: Circadian rhythm is an endogenous and self-sustained timing system, responsible for the coordination of daily processes in 24-h timescale. It is regulated by an endogenous molecular clock, which is sensitive to external cues as light and food. We have previously shown that grape seed proanthocyanidins extract (GSPE) regulates the hepatic molecular clock. Moreover, GSPE is known to interact with some microRNAs (miRNAs). Therefore, the aim of this study was to evaluate if the activity of GSPE as modulator of hepatic clock genes could be mediated by miRNAs.250 mg/kg of GSPE was administered to Wistar rats before a 6-hour jet lag and sacrificed at different time points. GSPE modulated both expression of Bmal1 and miR-27b-3p in the liver. Cosinor-based analysis revealed that both Bmal1 and miR-27b-3p expression followed a circadian rhythm, a negative interaction between them, and the role of GSPE adjusting the hepatic peripheral clock via miRNA. Additionally, in vitro studies showed that Bmal1 was sensitive to GSPE (25 mg/L). However, this effect was independent of miR-27b-3p.miRNA regulation of peripheral clocks via GSPE may be part of a complex mechanism that involves the crosstalk with the central system rather than a direct effect. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
    Thematic Areas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat francesca.manocchio@urv.cat francesca.manocchio@urv.cat francesca.manocchio@urv.cat manuel.suarez@urv.cat franciscaisabel.bravo@urv.cat anna.arola@urv.cat begona.muguerza@urv.cat
    Author identifier: 0000-0003-0122-8253 0000-0002-6468-3088 0000-0001-6529-1345 0000-0001-7384-8588
    Record's date: 2024-09-21
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://onlinelibrary.wiley.com/doi/full/10.1002/mnfr.202200443
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Molecular Nutrition & Food Research. 66 (23): e2200443-e2200443
    APA: Manocchio F; Soliz-Rueda JR; Ribas-Latre A; Bravo FI; Arola-Arnal A; Suarez M; Muguerza B (2022). Grape Seed Proanthocyanidins Modulate the Hepatic Molecular Clock via MicroRNAs. Molecular Nutrition & Food Research, 66(23), e2200443-e2200443. DOI: 10.1002/mnfr.202200443
    Article's DOI: 10.1002/mnfr.202200443
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Biotechnology,Food Science,Food Science & Technology
    Polyphenols
    Peripheral molecular clock
    Microrna
    Dose-dependent manner
    Cosinor-based rhythmometry
    Circadian rhythm
    rhythm
    rats
    polyphenols
    peripheral molecular clock
    mir-33a
    mir-122
    microrna
    metabolism
    healthy
    expression
    cosinor-based rhythmometry
    bmal1
    association
    Saúde coletiva
    Química
    Nutrição
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science & technology
    Food science
    Farmacia
    Educação física
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biotechnology
    Astronomia / física
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