Articles producció científica> Bioquímica i Biotecnologia

A mixture of four dietary fibres ameliorates adiposity and improves metabolic profile and intestinal health in cafeteria-fed obese rats: an integrative multi-omics approach

  • Identification data

    Identifier: imarina:9286869
    Authors:
    Estanyol-Torres, NuriaDomenech-Coca, CristinaGonzalez-Dominguez, RaulMinarro, AntonioReverter, FerranAntonio Moreno-Munoz, JoseJimenez, JesusMartin-Palomas, ManelCastellano-Escuder, PolMostafa, HamzaGarcia-Vallve, SantiAbasolo, NereaRodriguez, Miguel ATorrell, Helenadel Bas, Josep MSanchez-Pla, AlexCaimari, AntoniMas-Capdevila, AnnaAndres-Lacueva, CristinaCrescenti, Anna
    Abstract:
    The aim of this study was to assess the effects of a mixture of four dietary fibers on obese rats. Four groups of male Wistar rats were fed with either standard chow (STD) or cafeteria diet (CAF) and were orally supplemented with either fibre mixture (2 g kg−1 of body weight) (STD+F or CAF+F groups) or vehicle (STD+VH or CAF+VH groups). We studied a wide number of biometric, biochemical, transcriptomic, metagenomic and metabolomic variables and applied an integrative multivariate approach based on multiple factor analysis and Pearson's correlation analysis. A significant reduction in body weight, adiposity, HbA1c and HDL-cholesterol serum levels, and colon MPO activity was observed, whereas cecal weight and small intestine length:weight ratio were significantly increased in F-treated groups compared to control animals. CAF+F rats displayed a significant enhancement in energy expenditure, fat oxidation and fresh stool weight, and a significant reduction in adiponectin and LPS serum levels, compared to control group. Animals in STD+F group showed reduced serum LDL-cholesterol levels and a significant reduction in total cholesterol levels in the liver compared to STF+VH group. The intervention effect was reflected at the metabolomic (i.e., production of short-chain fatty acids, phenolic acids, and amino acids), metagenomic (i.e., modulation of Ruminococcus and Lactobacillus genus) and transcriptomic (i.e., expression of tight junctions and proteolysis) levels. Altogether, our integrative multi-omics approach highlights the potential of supplementation with a mixture of fibers to ameliorate the impairments triggered by obesity in terms of adiposity, metabolic profile, and intestinal health.
  • Others:

    Author, as appears in the article.: Estanyol-Torres, Nuria; Domenech-Coca, Cristina; Gonzalez-Dominguez, Raul; Minarro, Antonio; Reverter, Ferran; Antonio Moreno-Munoz, Jose; Jimenez, Jesus; Martin-Palomas, Manel; Castellano-Escuder, Pol; Mostafa, Hamza; Garcia-Vallve, Santi; Abasolo, Nerea; Rodriguez, Miguel A; Torrell, Helena; del Bas, Josep M; Sanchez-Pla, Alex; Caimari, Antoni; Mas-Capdevila, Anna; Andres-Lacueva, Cristina; Crescenti, Anna
    Department: Bioquímica i Biotecnologia
    URV's Author/s: ABASOLO ZABALO, NEREA / DOMENECH COCA, CRISTINA / Garcia Vallve, Santiago / Rodríguez Merayo, María Araceli
    Keywords: Rats, wistar Rats Obesity Multi-omics Metabolome Metabolic syndrome Male Intestinal health Gut microbiota Dietary fiber Diet-induced obesity Cholesterol Animals Adiposity multi-omics model mice metabolic syndrome markers lipopolysaccharide intestinal health inflammation endotoxemia disease diet-induced obesity concurrent adiposity
    Abstract: The aim of this study was to assess the effects of a mixture of four dietary fibers on obese rats. Four groups of male Wistar rats were fed with either standard chow (STD) or cafeteria diet (CAF) and were orally supplemented with either fibre mixture (2 g kg−1 of body weight) (STD+F or CAF+F groups) or vehicle (STD+VH or CAF+VH groups). We studied a wide number of biometric, biochemical, transcriptomic, metagenomic and metabolomic variables and applied an integrative multivariate approach based on multiple factor analysis and Pearson's correlation analysis. A significant reduction in body weight, adiposity, HbA1c and HDL-cholesterol serum levels, and colon MPO activity was observed, whereas cecal weight and small intestine length:weight ratio were significantly increased in F-treated groups compared to control animals. CAF+F rats displayed a significant enhancement in energy expenditure, fat oxidation and fresh stool weight, and a significant reduction in adiponectin and LPS serum levels, compared to control group. Animals in STD+F group showed reduced serum LDL-cholesterol levels and a significant reduction in total cholesterol levels in the liver compared to STF+VH group. The intervention effect was reflected at the metabolomic (i.e., production of short-chain fatty acids, phenolic acids, and amino acids), metagenomic (i.e., modulation of Ruminococcus and Lactobacillus genus) and transcriptomic (i.e., expression of tight junctions and proteolysis) levels. Altogether, our integrative multi-omics approach highlights the potential of supplementation with a mixture of fibers to ameliorate the impairments triggered by obesity in terms of adiposity, metabolic profile, and intestinal health.
    Thematic Areas: Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Molecular biology Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Enfermagem Endocrinology, diabetes and metabolism Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biochemistry & molecular biology Biochemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: santi.garcia-vallve@urv.cat araceli.rodriguez@urv.cat araceli.rodriguez@urv.cat
    Author identifier: 0000-0002-0348-7497 0000-0002-9456-6528 0000-0002-9456-6528
    Record's date: 2024-08-03
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/pii/S0955286322002522
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Journal Of Nutritional Biochemistry. 111 109184-
    APA: Estanyol-Torres, Nuria; Domenech-Coca, Cristina; Gonzalez-Dominguez, Raul; Minarro, Antonio; Reverter, Ferran; Antonio Moreno-Munoz, Jose; Jimenez, Je (2023). A mixture of four dietary fibres ameliorates adiposity and improves metabolic profile and intestinal health in cafeteria-fed obese rats: an integrative multi-omics approach. Journal Of Nutritional Biochemistry, 111(), 109184-. DOI: 10.1016/j.jnutbio.2022.109184
    Article's DOI: 10.1016/j.jnutbio.2022.109184
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry,Biochemistry & Molecular Biology,Clinical Biochemistry,Endocrinology, Diabetes and Metabolism,Molecular Biology,Nutrition & Dietetics,Nutrition and Dietetics
    Rats, wistar
    Rats
    Obesity
    Multi-omics
    Metabolome
    Metabolic syndrome
    Male
    Intestinal health
    Gut microbiota
    Dietary fiber
    Diet-induced obesity
    Cholesterol
    Animals
    Adiposity
    multi-omics
    model
    mice
    metabolic syndrome
    markers
    lipopolysaccharide
    intestinal health
    inflammation
    endotoxemia
    disease
    diet-induced obesity
    concurrent
    adiposity
    Saúde coletiva
    Química
    Odontología
    Nutrition and dietetics
    Nutrition & dietetics
    Nutrição
    Molecular biology
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Farmacia
    Enfermagem
    Endocrinology, diabetes and metabolism
    Educação física
    Clinical biochemistry
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biochemistry & molecular biology
    Biochemistry
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