Author, as appears in the article.: Fernandez-Bravo, Ana; Vega-Sanchez, Vicente; Perez-Cataluna, Alba; Latif-Eugenin, Fadua; Beaz-Hidalgo, Roxana; Martinez-Murcia, Antonio; Soriano-Vargas, Edgardo; Alejandro Cabrero-Martinez, Omar; Castro-Escarpulli, Graciela; Jose Figueras, Maria;
Department: Ciències Mèdiques Bàsiques
URV's Author/s: BEAZ HIDALGO, ROXANA / Fernández Bravo, Ana / Figueras Salvat, María Josefa / PÉREZ CATALUÑA, ALBA
Keywords: Virulence genes Taxonomy Spp. Sp nov. Salmonicida Isddh Identification Genus aeromonas Genome sequence Escherichia-coli Diversity Core genomes Citrate synthase Ani Aeromonas lusitana
Abstract: The species Aeromonas lusitana was first described in 2016 with five strains recovered from untreated water and vegetables from Portugal. Since then, no further records exist of this species. During a surveillance study on the presence of Aeromonas in fish farms in Mexico, a new strain (ESV351) of the mentioned species isolated from a rainbow trout was recovered. It was identified because it clustered phylogenetically with the type strain of A. lusitana based on the analysis of the ipoD gene sequences. In the present study, phenotypic characteristics, antimicrobial resistance profiles, and the presence of putative virulence genes of this novel strain (ESV-351) were determined in parallel to the five isolates from the original species description. Phenotypic differential characteristics exhibited by A. lusitana ESV-351 depicted an evident similarity to the characteristics exhibited by the other evaluated strains. However, the novel strain was positive for the production of indole using conventional methods, while the rest of the strains, including the type strain, were negative for its production. Furthermore, intermediate resistance to ampicillin, amoxicillin-clavulanic acid and cephalothin was detected in both the novel and the type strain. Five different virulence-related genes were detected in the novel strain and in the previously described strains, with the type strain exhibiting the highest number of virulence-related genes. In addition to this, the genome of the novel strain (ESV-351) was sequenced and compared with the genomes from the type strain (A. lusitana CECT 7828(T)) and other Aeromonas spp. The genomic analysis defined Aeromonas tecta as the closest species to A. lusitana with a highly similar number of predicted proteins. The genomic size, the number of protein-encoding genes and the number of different tRNAs, among other characteristics, make it possible to propose that the ESV-351 strain could potentially have the capacity to adapt to different environments. Genome comparison of the ESV-351 strain with the type strain revealed that both possess a similar sequence of the citrate synthase gene. In addition to this finding, the chromosomal region containing the citrate synthase locus of the novel strain exhibits some similarity to the chromosomal region in the genome of the A. hydrophila type strain and other known human pathogens, such as Vibrio cholerae. This could suggest a possible virulence role for the citrate synthase gene in A. lusitana (ESV-351).
Thematic Areas: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: ana.fernandez@urv.cat mariajose.figueras@urv.cat
Author identifier: 0000-0001-7637-5958 0000-0002-2268-8980
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2076-0817/11/11/1299
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Pathogens. 11 (11):
APA: Fernandez-Bravo, Ana; Vega-Sanchez, Vicente; Perez-Cataluna, Alba; Latif-Eugenin, Fadua; Beaz-Hidalgo, Roxana; Martinez-Murcia, Antonio; Soriano-Varga (2022). First Record of the Rare Species Aeromonas lusitana from Rainbow Trout (Oncorhynchus mykiss, Walbaum): Comparative Analysis with the Existing Strains. Pathogens, 11(11), -. DOI: 10.3390/pathogens11111299
Article's DOI: 10.3390/pathogens11111299
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV