Author, as appears in the article.: Moabelo, Koena L; Lerga, Teresa M; Jauset-Rubio, Miriam; Sibuyi, Nicole R S; O'Sullivan, Ciara K; Meyer, Mervin; Madiehe, Abram M
Department: Enginyeria Química
URV's Author/s: Jauset Rubio, Miriam / Mairal Lerga, Teresa / O'SULLIVAN, CIARA KATHLEEN
Keywords: Type 2 diabetes mellitus Retinol-binding protein 4 Insulin-resistance Gold nanoparticles Diagnosis Colorimetric Biosensing Aptasensor visual detection type 2 diabetes mellitus surface science sensitive detection retinol-binding-protein-4 rapid detection gold nanoparticles early-diagnosis dna diagnosis colorimetric aptasensor colorimetric biosensing assay aptasensor
Abstract: Retinol-binding protein 4 (RBP4) has been implicated in insulin resistance in rodents and humans with obesity and T2DM, making it a potential biomarker for the early diagnosis of T2DM. However, diagnostic tools for low-level detection of RBP4 are still lagging behind. Therefore, there is an urgent need for the development of T2DM diagnostics that are rapid, cost-effective and that can be used at the point-of-care (POC). Recently, nano-enabled biosensors integrating highly selective optical detection techniques and specificity of aptamers have been widely developed for the rapid detection of various targets. This study reports on the development of a rapid gold nanoparticles (AuNPs)-based aptasensor for the detection of RBP4. The retinol-binding protein aptamer (RBP-A) is adsorbed on the surface of the AuNPs through van der Waals and hydrophobic interactions, stabilizing the AuNPs against sodium chloride (NaCl)-induced aggregation. Upon the addition of RBP4, the RBP-A binds to RBP4 and detaches from the surface of the AuNPs, leaving the AuNPs unprotected. Addition of NaCl causes aggregation of AuNPs, leading to a visible colour change of the AuNPs solution from ruby red to purple/blue. The test result was available within 5 min and the assay had a limit of detection of 90.76 ± 2.81 nM. This study demonstrates the successful development of a simple yet effective, specific, and colorimetric rapid assay for RBP4 detection.
Thematic Areas: Química Nanoscience & nanotechnology Medicine (miscellaneous) Materiais Instruments & instrumentation Instrumentation Engineering (miscellaneous) Clinical biochemistry Ciencias sociales Ciências biológicas iii Ciências biológicas i Chemistry, analytical Biotecnología Biotechnology Biomedical engineering Astronomia / física Analytical chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: teresa.mairal@urv.cat miriam.jauset@urv.cat
Author identifier: 0000-0002-9943-6132
Record's date: 2024-10-26
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2079-6374/12/12/1061
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Biosensors. 12 (12): 1061-
APA: Moabelo, Koena L; Lerga, Teresa M; Jauset-Rubio, Miriam; Sibuyi, Nicole R S; O'Sullivan, Ciara K; Meyer, Mervin; Madiehe, Abram M (2022). A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4. Biosensors, 12(12), 1061-. DOI: 10.3390/bios12121061
Article's DOI: 10.3390/bios12121061
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications