Articles producció científica> Ciències Mèdiques Bàsiques

Potential Pathogenicity of Aeromonas spp. Recovered in River Water, Soil, and Vegetation from a Natural Recreational Area

  • Identification data

    Identifier: imarina:9287667
  • Authors:

    Guerra, Roberto M.
    Damian Maleno, Francisco
    Jose Figueras, Maria
    Pujol-Bajador, Isabel
    Fernandez-Bravo, Ana
    Pathogens
    10.3390/pathogens11111382
    Pathogens. 11 (11):
  • Others:

    Author, as appears in the article.: Guerra, Roberto M.; Damian Maleno, Francisco; Jose Figueras, Maria; Pujol-Bajador, Isabel; Fernandez-Bravo, Ana;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Fernández Bravo, Ana / Figueras Salvat, María Josefa / Pujol Bajador, Isabel
    Keywords: Virulence factors Strains River water Phenotypic identification Pathogenicity Macrophages Lateral flagella Iii secretion system Genus aeromonas Genes Environmental sources Antimicrobial susceptibility Antibiotic-resistance Aeromonas
    Abstract: The genus Aeromonas is widely distributed in aquatic environments and is recognized as a potential human pathogen. Some Aeromonas species are able to cause a wide spectrum of diseases, mainly gastroenteritis, skin and soft-tissue infections, bacteremia, and sepsis. Currently, untreated river water is used for irrigation and recreational purposes. In this study, the Aeromonas spp. present in a river recreational environment was investigated by quantifying its presence in water, soil, and vegetation using three techniques: qPCR, plate counting in selective ADA medium, and Most Probable Number, in parallel. The presence of clones in the three types of samples was elucidated through genotyping with the ERIC-PCR technique, whereas the identification of the isolated Aeromonas was carried out by sequencing the rpoD gene. Finally, the pathogenic potential of some of the strains was explored by studying the presence and expression of virulence genes characteristic of the genus, their antimicrobial susceptibility profile, as well as the quantification of their cell damage and intracellular survival in an in vitro macrophages infection model. The results showed the presence of Aeromonas in all samples with the three quantification methods, with Aeromonas popoffii being the most prevalent species. The presence of strains with the same genotype (ERIC-PCR) was also confirmed in different samples. Some of the strains showed a high level of cell damage and intracellular bacterial survival, as well as the presence of various virulence factors. Furthermore, these strains showed resistance to some of the antibiotics tested and used therapeutically in both humans and animals. These results indicate that the presence of Aeromonas in this environment may represent a biosanitary risk that could be a public health problem.
    Thematic Areas: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: ana.fernandez@urv.cat isabel.pujol@urv.cat mariajose.figueras@urv.cat
    Author identifier: 0000-0001-7637-5958 0000-0002-2268-8980
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2076-0817/11/11/1382
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Pathogens. 11 (11):
    APA: Guerra, Roberto M.; Damian Maleno, Francisco; Jose Figueras, Maria; Pujol-Bajador, Isabel; Fernandez-Bravo, Ana; (2022). Potential Pathogenicity of Aeromonas spp. Recovered in River Water, Soil, and Vegetation from a Natural Recreational Area. Pathogens, 11(11), -. DOI: 10.3390/pathogens11111382
    Article's DOI: 10.3390/pathogens11111382
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Immunology and Allergy,Immunology and Microbiology (Miscellaneous),Infectious Diseases,Microbiology,Microbiology (Medical),Molecular Biology
    Virulence factors
    Strains
    River water
    Phenotypic identification
    Pathogenicity
    Macrophages
    Lateral flagella
    Iii secretion system
    Genus aeromonas
    Genes
    Environmental sources
    Antimicrobial susceptibility
    Antibiotic-resistance
    Aeromonas
    Molecular biology
    Microbiology (medical)
    Microbiology
    Infectious diseases
    Immunology and microbiology (miscellaneous)
    Immunology and microbiology (all)
    Immunology and allergy
    General immunology and microbiology
    Farmacia
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