Author, as appears in the article.: Cuesta, Natalia; Fernandez-Veledo, Sonia; Punzon, Carmen; Moreno, Cristobal; Barrocal, Beatriz; Sreeramkumar, Vinatha; Desco, Manuel; Fresno, Manuel;
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Fernandez Veledo, Sonia
Abstract: Understanding the signaling cascades that govern adipocyte metabolism and differentiation is necessary for the development of therapies for obesity. Toll-like receptors (TLRs) are key mediators in adipogenesis, but their specific role is not completely understood. In this study, siRNA knockdown of Tlr2 in 3T3-L1 cells allowed them to differentiate more efficiently into adipocytes, whereas the opposite was observed for the knockdown of Tlr4. At the same time, we show that TLR2 knock-out mice spontaneously developed mature-onset obesity and insulin resistance. Besides a higher incidence of hyperplasia and hypertrophy in white adipose tissue (WAT), we found a significantly increased number of adipocyte precursor cells in TLR2?/? mice compared to TLR4?/? mice. Interestingly, genetic inactivation of Tlr4 in TLR2?/? mice reverted their increased adiposity, insulin resistance, and restored normal levels of adipocyte precursor cells. These findings provide evidence that TLR2 and TLR4 play opposing roles in WAT homeostasis and point to the existence of cross-regulation among TLR2 and TLR4 during adipocyte differentiation both in vitro and in vivo.
Thematic Areas: Astronomia / física Biochemistry & molecular biology Biodiversidade Biotecnología Catalysis Chemistry, multidisciplinary Ciência da computação Ciência de alimentos Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Computer science applications Educação física Engenharias i Engenharias ii Engenharias iv Farmacia Geociências Inorganic chemistry Interdisciplinar Materiais Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (miscellaneous) Molecular biology Nutrição Odontología Organic chemistry Physical and theoretical chemistry Psicología Química Saúde coletiva Spectroscopy Zootecnia / recursos pesqueiros
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: sonia.fernandez@urv.cat
Author identifier: 0000-0003-2906-3788
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://mdpi-res.com/d_attachment/ijms/ijms-23-15682/article_deploy/ijms-23-15682-v2.pdf?version=1671672468
Papper original source: International Journal Of Molecular Sciences. 23 (24): 15682-
APA: Cuesta, Natalia; Fernandez-Veledo, Sonia; Punzon, Carmen; Moreno, Cristobal; Barrocal, Beatriz; Sreeramkumar, Vinatha; Desco, Manuel; Fresno, Manuel; (2022). Opposing Actions of TLR2 and TLR4 in Adipocyte Differentiation and Mature-Onset Obesity. International Journal Of Molecular Sciences, 23(24), 15682-. DOI: 10.3390/ijms232415682
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.3390/ijms232415682
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV