Author, as appears in the article.: Moure, Zaira; Lara, Noelia; Marin, Mercedes; Sola-Campoy, Pedro J; Bautista, Veronica; Gomez-Bertomeu, Frederic; Gomez-Dominguez, Cristina; Perez-Vazquez, Maria; Aracil, Belen; Campos, Jose; Cercenado, Emilia; Oteo-Iglesias, Jesus
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Gomez Bertomeu, Frederic-Francesc
Keywords: Whole genome sequencing Transferable resistance Surveillance Spain Ribosomal-rna Poxta Plasmids Optra Mutation Multilocus sequence typing Molecular epidemiology Male Linezolid resistance Linezolid Humans Gram-positive bacterial infections Genome, bacterial Gene transfer, horizontal Female Faecium Faecalis Enterococcus faecium Enterococcus faecalis Enterococcus Drug resistance, bacterial Anti-bacterial agents Aged
Abstract: The emergence of linezolid-resistant Enterococcus spp. (LRE) due to transferable resistance determinants is a matter of concern. To understand the contribution of the plasmid-encoded optrA and poxtA genes to the emergence of LRE, clinical isolates from different Spanish hospitals submitted to the Spanish Reference Laboratory from 2015-2018 were analysed. Linezolid resistance mechanisms were screened in all isolates by PCR and sequencing. Genetic relatedness of Enterococcus spp. carrying optrA and poxtA was studied by PFGE and MLST. Antimicrobial susceptibility was tested by broth microdilution using EUCAST standards. A total of 97 LRE isolates were studied, in 94 (96.9%) of which at least one resistance determinant was detected; 84/97 isolates (86.6%) presented a single resistance mechanism as follows: 45/84 (53.6%) carried the optrA gene, 38/84 (45.2%) carried the G2576T mutation and 1/84 (1.2%) carried the poxtA gene. In addition, 5/97 isolates (5.2%) carried both optrA and the G2576T mutation and 5/97 (5.2%) carried both optrA and poxtA . The optrA gene was more frequent in Enterococcus faecalis (83.6%) than Enterococcus faecium (11.1%) and was mainly associated with community-acquired urinary tract infections. Carriage of the poxtA gene was more frequent in E. faecium (13.9%) than E. faecalis (1.6%). Among the optrA-positive E. faecalis isolates, two main clusters were detected by PFGE. These two clusters belonged to ST585 and ST480 and were distributed throughout 11 and 6 Spanish provinces, respectively. This is the first description of LRE carrying the poxtA gene in Spain, including the co-existence of optrA and poxtA in five isolates. (C) 2020 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
Thematic Areas: Saúde coletiva Química Pharmacology (medical) Pharmacology & pharmacy Odontología Nutrição Microbiology (medical) Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases General medicine Farmacia Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Biodiversidade
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: frederic-francesc.gomez@urv.cat
Author identifier: 0000-0002-8039-2889
Record's date: 2025-02-19
Paper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: International Journal Of Antimicrobial Agents. 55 (6): 105977-
APA: Moure, Zaira; Lara, Noelia; Marin, Mercedes; Sola-Campoy, Pedro J; Bautista, Veronica; Gomez-Bertomeu, Frederic; Gomez-Dominguez, Cristina; Perez-Vazq (2020). Interregional spread in Spain of linezolid-resistant Enterococcus spp. isolates carrying the optrA and poxtA genes. International Journal Of Antimicrobial Agents, 55(6), 105977-. DOI: 10.1016/j.ijantimicag.2020.105977
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV