Articles producció científica> Medicina i Cirurgia

DDR1 and Its Ligand, Collagen IV, Are Involved in In Vitro Oligodendrocyte Maturation

  • Identification data

    Identifier: imarina:9290113
    Authors:
    Silva, MEHernández-Andrade, MAbasolo, NEspinoza-Cruells, CMansilla, JBReyes, CRAranda, SEsteban, YRodriguez-Calvo, RMartorell, LMuntane, GRivera, FJVilella, E
    Abstract:
    Discoidin domain receptor 1 (DDR1) is a tyrosine kinase receptor expressed in epithelial cells from different tissues in which collagen binding activates pleiotropic functions. In the brain, DDR1 is mainly expressed in oligodendrocytes (OLs), the function of which is unclear. Whether collagen can activate DDR1 in OLs has not been studied. Here, we assessed the expression of DDR1 during in vitro OL differentiation, including collagen IV incubation, and the capability of collagen IV to induce DDR1 phosphorylation. Experiments were performed using two in vitro models of OL differentiation: OLs derived from adult rat neural stem cells (NSCs) and the HOG16 human oligodendroglial cell line. Immunocytofluorescence, western blotting, and ELISA were performed to analyze these questions. The differentiation of OLs from NSCs was addressed using oligodendrocyte transcription factor 2 (Olig2) and myelin basic protein (MBP). In HOG16 OLs, collagen IV induced DDR1 phosphorylation through slow and sustained kinetics. In NSC-derived OLs, DDR1 was found in a high proportion of differentiating cells (MBP+/Olig2+), but its protein expression was decreased in later stages. The addition of collagen IV did not change the number of DDR1+/MBP+ cells but did accelerate OL branching. Here, we provide the first demonstration that collagen IV mediates the phosphorylation of DDR1 in HOG16 cells and that the in vitro co-expression of DDR1 and MBP is associated with accelerated branching during the differentiation of primary OLs.
  • Others:

    Author, as appears in the article.: Silva, ME; Hernández-Andrade, M; Abasolo, N; Espinoza-Cruells, C; Mansilla, JB; Reyes, CR; Aranda, S; Esteban, Y; Rodriguez-Calvo, R; Martorell, L; Muntane, G; Rivera, FJ; Vilella, E
    Department: Medicina i Cirurgia
    URV's Author/s: ABASOLO ZABALO, NEREA / Aranda Castel, Selena / Martorell Bonet, Lourdes / Muntané Medina, Gerard / Rodríguez Calvo, Ricardo / Vilella Cuadrada, Elisabet
    Keywords: Tyrosine kinase receptor Phosphorylation Oligodendrocytes Olig2 Mbp Domain receptor 1 Ddr1 Collagen iv Branching tyrosine kinase receptor protein-coupled receptors phosphorylation peripheral-nerve myelination oligodendrocytes olig2 mechanisms mbp isoform gene expression discoidin-domain-receptor-1 differentiation ddr1 cells branching
    Abstract: Discoidin domain receptor 1 (DDR1) is a tyrosine kinase receptor expressed in epithelial cells from different tissues in which collagen binding activates pleiotropic functions. In the brain, DDR1 is mainly expressed in oligodendrocytes (OLs), the function of which is unclear. Whether collagen can activate DDR1 in OLs has not been studied. Here, we assessed the expression of DDR1 during in vitro OL differentiation, including collagen IV incubation, and the capability of collagen IV to induce DDR1 phosphorylation. Experiments were performed using two in vitro models of OL differentiation: OLs derived from adult rat neural stem cells (NSCs) and the HOG16 human oligodendroglial cell line. Immunocytofluorescence, western blotting, and ELISA were performed to analyze these questions. The differentiation of OLs from NSCs was addressed using oligodendrocyte transcription factor 2 (Olig2) and myelin basic protein (MBP). In HOG16 OLs, collagen IV induced DDR1 phosphorylation through slow and sustained kinetics. In NSC-derived OLs, DDR1 was found in a high proportion of differentiating cells (MBP+/Olig2+), but its protein expression was decreased in later stages. The addition of collagen IV did not change the number of DDR1+/MBP+ cells but did accelerate OL branching. Here, we provide the first demonstration that collagen IV mediates the phosphorylation of DDR1 in HOG16 cells and that the in vitro co-expression of DDR1 and MBP is associated with accelerated branching during the differentiation of primary OLs.
    Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: gerard.muntane@urv.cat ricardo.rodriguez@urv.cat lourdes.martorell@urv.cat elisabet.vilella@urv.cat selena.aranda@estudiants.urv.cat
    Author identifier: 0000-0003-4999-2197 0000-0002-1887-5919
    Record's date: 2024-08-03
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1422-0067/24/2/1742
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: International Journal Of Molecular Sciences. 24 (2):
    APA: Silva, ME; Hernández-Andrade, M; Abasolo, N; Espinoza-Cruells, C; Mansilla, JB; Reyes, CR; Aranda, S; Esteban, Y; Rodriguez-Calvo, R; Martorell, L; Mu (2023). DDR1 and Its Ligand, Collagen IV, Are Involved in In Vitro Oligodendrocyte Maturation. International Journal Of Molecular Sciences, 24(2), -. DOI: 10.3390/ijms24021742
    Article's DOI: 10.3390/ijms24021742
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Tyrosine kinase receptor
    Phosphorylation
    Oligodendrocytes
    Olig2
    Mbp
    Domain receptor 1
    Ddr1
    Collagen iv
    Branching
    tyrosine kinase receptor
    protein-coupled receptors
    phosphorylation
    peripheral-nerve myelination
    oligodendrocytes
    olig2
    mechanisms
    mbp
    isoform
    gene
    expression
    discoidin-domain-receptor-1
    differentiation
    ddr1
    cells
    branching
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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