Author, as appears in the article.: Silva, ME; Hernández-Andrade, M; Abasolo, N; Espinoza-Cruells, C; Mansilla, JB; Reyes, CR; Aranda, S; Esteban, Y; Rodriguez-Calvo, R; Martorell, L; Muntane, G; Rivera, FJ; Vilella, E
Department: Medicina i Cirurgia
URV's Author/s: ABASOLO ZABALO, NEREA / Aranda Castel, Selena / Martorell Bonet, Lourdes / Muntané Medina, Gerard / Rodríguez Calvo, Ricardo / Vilella Cuadrada, Elisabet
Keywords: Tyrosine kinase receptor Phosphorylation Oligodendrocytes Olig2 Mbp Domain receptor 1 Ddr1 Collagen iv Branching tyrosine kinase receptor protein-coupled receptors phosphorylation peripheral-nerve myelination oligodendrocytes olig2 mechanisms mbp isoform gene expression discoidin-domain-receptor-1 differentiation ddr1 cells branching
Abstract: Discoidin domain receptor 1 (DDR1) is a tyrosine kinase receptor expressed in epithelial cells from different tissues in which collagen binding activates pleiotropic functions. In the brain, DDR1 is mainly expressed in oligodendrocytes (OLs), the function of which is unclear. Whether collagen can activate DDR1 in OLs has not been studied. Here, we assessed the expression of DDR1 during in vitro OL differentiation, including collagen IV incubation, and the capability of collagen IV to induce DDR1 phosphorylation. Experiments were performed using two in vitro models of OL differentiation: OLs derived from adult rat neural stem cells (NSCs) and the HOG16 human oligodendroglial cell line. Immunocytofluorescence, western blotting, and ELISA were performed to analyze these questions. The differentiation of OLs from NSCs was addressed using oligodendrocyte transcription factor 2 (Olig2) and myelin basic protein (MBP). In HOG16 OLs, collagen IV induced DDR1 phosphorylation through slow and sustained kinetics. In NSC-derived OLs, DDR1 was found in a high proportion of differentiating cells (MBP+/Olig2+), but its protein expression was decreased in later stages. The addition of collagen IV did not change the number of DDR1+/MBP+ cells but did accelerate OL branching. Here, we provide the first demonstration that collagen IV mediates the phosphorylation of DDR1 in HOG16 cells and that the in vitro co-expression of DDR1 and MBP is associated with accelerated branching during the differentiation of primary OLs.
Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: gerard.muntane@urv.cat ricardo.rodriguez@urv.cat lourdes.martorell@urv.cat elisabet.vilella@urv.cat selena.aranda@estudiants.urv.cat
Author identifier: 0000-0003-4999-2197 0000-0002-1887-5919
Record's date: 2024-08-03
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/1422-0067/24/2/1742
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: International Journal Of Molecular Sciences. 24 (2):
APA: Silva, ME; Hernández-Andrade, M; Abasolo, N; Espinoza-Cruells, C; Mansilla, JB; Reyes, CR; Aranda, S; Esteban, Y; Rodriguez-Calvo, R; Martorell, L; Mu (2023). DDR1 and Its Ligand, Collagen IV, Are Involved in In Vitro Oligodendrocyte Maturation. International Journal Of Molecular Sciences, 24(2), -. DOI: 10.3390/ijms24021742
Article's DOI: 10.3390/ijms24021742
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications