Author, as appears in the article.: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; Gil-Solsona, R
Department: Ciències Mèdiques Bàsiques
URV's Author/s: García Sayago, Francisco / Marquès Bueno, Montserrat
Keywords: Suspect screening Non-target screening Lc-hrms Human tissue Human biomonitoring Exposome Bead beating suspect screening non-target screening lc-hrms human biomonitoring bead beating
Abstract: Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
Thematic Areas: Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medical laboratory technology Interdisciplinar Engenharias iii Engenharias ii Clinical biochemistry Ciências biológicas iii Ciências ambientais Ciência de alimentos
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: francisco.garcia@urv.cat montserrat.marques@urv.cat
Author identifier: 0000-0003-0594-8370 0000-0001-6302-8578
Record's date: 2024-08-03
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://methods-x.com/article/S2215-0161(23)00072-9/fulltext
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Methodsx. 10 (102069):
APA: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; (2023). Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants. Methodsx, 10(102069), -. DOI: 10.1016/j.mex.2023.102069
Article's DOI: 10.1016/j.mex.2023.102069
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV