Author, as appears in the article.: Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda;
Department: Enginyeria Química
URV's Author/s: Constantí Garriga, Magdalena / GUNASEKARAN, VIJAYALAKSHMI
Keywords: Strain Sp nov. Pathway Mtbe Metaproteome Inhalation exposure Hydrocarbons Herbaspirillum-chlorophenolicum Etbe Degradation Biodegradation Bacterial consortium 2d-dige
Abstract: A bacterial consortium capable of degrading ethyl tert-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. Arthrobacter sp., Herbaspirillum sp., Pseudacidovorax sp., Pseudomonas sp., and Xanthomonas sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). Xanthomonas sp. and Pseudomonas sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community.
Thematic Areas: Virology Microbiology (medical) Microbiology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: magdalena.constanti@urv.cat
Author identifier: 0000-0002-3547-4199
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2076-2607/10/12/2331
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Microorganisms. 10 (12):
APA: Gunasekaran, Vijayalakshmi; Canela, Nuria; Constanti, Magda; (2022). Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium. Microorganisms, 10(12), -. DOI: 10.3390/microorganisms10122331
Article's DOI: 10.3390/microorganisms10122331
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV