Articles producció científica> Química Física i Inorgànica

Functionalized Gold Nanoclusters Promote Stress Response in COS-7 Cells

  • Identification data

    Identifier: imarina:9293186
    Authors:
    Linklater, DPLe Guével, XKosyer, ERubanov, SBryant, GHanssen, EBaulin, VAPereiro, EPerera, PGWandiyanto, JVAngulo, AJuodkazis, SIvanova, EP
    Abstract:
    Ultrasmall gold nanoclusters (AuNC) show great promise for application in theranostics due to their unique optical and physicochemical properties; however, the associated nanotoxicology concerns need to be carefully considered because of their high diffusion across the cellular barrier. Herein, new insights into the role of surface modification of 2 nm AuNC on their toxicity with impact on the metabolism of COS-7 fibroblast-like cells are revealed. AuNCs are chemically modified with either a monodentate-thiolated molecule (AuNC-MHA) or a modified-bidentate sulfobetaine zwitterionic molecule (AuNC-ZwBuEt). Uptake and localization inside fibroblasts and the resultant influence on cell ultrastructure are carefully evaluated using scanning transmission electron microscopy (STEM) and cryo-soft-X-ray tomography (cryo-SXT). At concentrations of >= 25 mu g Au mL(-1), AuNC-ZwBuEt are cytotoxic toward COS-7 cells and are observed to cross the nuclear membrane. Cryo-SXT analysis shows that fibroblasts develop an acute stress response in the form of swollen mitochondria, nuclear membrane blebbing, and large cytoplasmic vacuoles as early as 1 h postexposure. By contrast, AuNC-MHA are not cytotoxic toward COS-7 cells. Endosomal escape and translocation of the AuNC-ZwBuEt into the nucleus and other organelles may be the cause for the observed cytotoxicity and highlight the need for further study of metal nanocluster-cell interactions.
  • Others:

    Author, as appears in the article.: Linklater, DP; Le Guével, X; Kosyer, E; Rubanov, S; Bryant, G; Hanssen, E; Baulin, VA; Pereiro, E; Perera, PG; Wandiyanto, JV; Angulo, A; Juodkazis, S; Ivanova, EP
    Department: Química Física i Inorgànica
    URV's Author/s: Baulin, Vladimir
    Keywords: Toxicity Size Shape Nanotoxicity Nanoparticles Metal nanoclusters Ligand Gold nanoclusters
    Abstract: Ultrasmall gold nanoclusters (AuNC) show great promise for application in theranostics due to their unique optical and physicochemical properties; however, the associated nanotoxicology concerns need to be carefully considered because of their high diffusion across the cellular barrier. Herein, new insights into the role of surface modification of 2 nm AuNC on their toxicity with impact on the metabolism of COS-7 fibroblast-like cells are revealed. AuNCs are chemically modified with either a monodentate-thiolated molecule (AuNC-MHA) or a modified-bidentate sulfobetaine zwitterionic molecule (AuNC-ZwBuEt). Uptake and localization inside fibroblasts and the resultant influence on cell ultrastructure are carefully evaluated using scanning transmission electron microscopy (STEM) and cryo-soft-X-ray tomography (cryo-SXT). At concentrations of >= 25 mu g Au mL(-1), AuNC-ZwBuEt are cytotoxic toward COS-7 cells and are observed to cross the nuclear membrane. Cryo-SXT analysis shows that fibroblasts develop an acute stress response in the form of swollen mitochondria, nuclear membrane blebbing, and large cytoplasmic vacuoles as early as 1 h postexposure. By contrast, AuNC-MHA are not cytotoxic toward COS-7 cells. Endosomal escape and translocation of the AuNC-ZwBuEt into the nucleus and other organelles may be the cause for the observed cytotoxicity and highlight the need for further study of metal nanocluster-cell interactions.
    Thematic Areas: Nanoscience & nanotechnology Medicine (miscellaneous) Materials science, biomaterials Engineering, biomedical Engineering (miscellaneous) Biomaterials Applied microbiology and biotechnology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: vladimir.baulin@urv.cat
    Author identifier: 0000-0003-2086-4271
    Record's date: 2024-08-03
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://onlinelibrary.wiley.com/doi/full/10.1002/anbr.202200102
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Advanced Nanobiomed Research. 3 (4):
    APA: Linklater, DP; Le Guével, X; Kosyer, E; Rubanov, S; Bryant, G; Hanssen, E; Baulin, VA; Pereiro, E; Perera, PG; Wandiyanto, JV; Angulo, A; Juodkazis, S (2023). Functionalized Gold Nanoclusters Promote Stress Response in COS-7 Cells. Advanced Nanobiomed Research, 3(4), -. DOI: 10.1002/anbr.202200102
    Article's DOI: 10.1002/anbr.202200102
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Engineering, Biomedical,Materials Science, Biomaterials,Nanoscience & Nanotechnology
    Toxicity
    Size
    Shape
    Nanotoxicity
    Nanoparticles
    Metal nanoclusters
    Ligand
    Gold nanoclusters
    Nanoscience & nanotechnology
    Medicine (miscellaneous)
    Materials science, biomaterials
    Engineering, biomedical
    Engineering (miscellaneous)
    Biomaterials
    Applied microbiology and biotechnology
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