Author, as appears in the article.: Molejon, Nerissa A; Lapada, Catherine M; Skouridou, Vasso; Rollon, Analiza P; El-Shahawi, Mohammed S; Bashammakh, Abdulaziz S; O'Sullivan, Ciara K
Department: Enginyeria Química
URV's Author/s: O'SULLIVAN, CIARA KATHLEEN / Skouridou, Vasoula
Keywords: Vibrio cholerae Next generation sequencing G-rich sequence motif Enterotoxigenic escherichia coli Dna aptamer Ab(5) enterotoxins Ab enterotoxins 5
Abstract: Cholera and milder diarrheal disease are caused by Vibrio cholerae and enterotoxigenic Escherichia coli and are still a prominent public health concern. Evaluation of suspicious isolates is essential for the rapid containment of acute diarrhea outbreaks or prevention of epidemic cholera. Existing detection techniques require expensive equipment, trained personnel and are time-consuming. Antibody-based methods are also available, but cost and stability issues can limit their applications for point-of-care testing. This study focused on the selection of single stranded DNA aptamers as simpler, more stable and more cost-effective alternatives to antibodies for the co-detection of AB5 toxins secreted by enterobacteria causing acute diarrheal infections. Cholera toxin and Escherichia coli heat-labile enterotoxin, the key toxigenicity biomarkers of these bacteria, were immobilized on magnetic beads and were used in a SELEX-based selection strategy. This led to the enrichment of sequences with a high % GC content and a dominant G-rich motif as revealed by Next Generation Sequencing. Enriched sequences were confirmed to fold into G-quadruplex structures and the binding of one of the most abundant candidates to the two enterotoxins was confirmed. Ongoing work is focused on the development of monitoring tools for potential environmental surveillance of epidemic choleraand milder diarrheal disease.Copyright © 2023. Published by Elsevier Inc.
Thematic Areas: Química Odontología Molecular biology Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Engenharias iv Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, analytical Cell biology Biotecnología Biophysics Biochemistry & molecular biology Biochemistry Biochemical research methods Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: vasoula.skouridou@urv.cat
Author identifier: 0000-0002-9712-5429
Record's date: 2024-10-26
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.sciencedirect.com/science/article/pii/S0003269723000830
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Analytical Biochemistry. 669 115118-115118
APA: Molejon, Nerissa A; Lapada, Catherine M; Skouridou, Vasso; Rollon, Analiza P; El-Shahawi, Mohammed S; Bashammakh, Abdulaziz S; O'Sullivan, Ciara K (2023). Selection of G-rich ssDNA aptamers for the detection of enterotoxins of the cholera toxin family. Analytical Biochemistry, 669(), 115118-115118. DOI: 10.1016/j.ab.2023.115118
Article's DOI: 10.1016/j.ab.2023.115118
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV