Articles producció científica> Ciències Mèdiques Bàsiques

Drosophila melanogaster Systemic Infection Model to Study Altered Virulence during Polymicrobial Infection by Aeromonas

  • Identification data

    Identifier: imarina:9295157
  • Authors:

    Robert, Alexandre
    Talagrand-Reboul, Emilie
    Figueras, Maria-Jose
    Ruimy, Raymond
    Boyer, Laurent
    Lamy, Brigitte
    Pathogens
    10.3390/pathogens12030405
    Pathogens. 12 (3):
  • Others:

    Author, as appears in the article.: Robert, Alexandre; Talagrand-Reboul, Emilie; Figueras, Maria-Jose; Ruimy, Raymond; Boyer, Laurent; Lamy, Brigitte;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Figueras Salvat, María Josefa
    Keywords: Pseudomonas-aeruginosa Polymicrobial infection Opportunistic pathogen Microbial interference Lessons Infection model Host Drosophila melanogaster Disease Bacteria Aeromonas
    Abstract: Background: Polymicrobial infections are complex infections associated with worse outcomes compared to monomicrobial infections. We need simple, fast, and cost-effective animal models to assess their still poorly known pathogenesis. Methods: We developed a Drosophila melanogaster polymicrobial infection model for opportunistic pathogens and assessed its capacity to discriminate the effects of bacterial mixtures taken from cases of human polymicrobial infections by Aeromonas strains. A systemic infection was obtained by needle pricking the dorsal thorax of the flies, and the fly survival was monitored over time. Different lineages of the flies were infected by a single strain or paired strains (strain ratio 1:1). Results: Individual strains killed more than 80% of the flies in 20 h. The course of infection could be altered with a microbial mix. The model could distinguish between the diverse effects (synergistic, antagonistic, and no difference) that resulted in a milder, more severe, or similar infection, depending on the paired strain considered. We then investigated the determinants of the effects. The effects were maintained in deficient fly lineages for the main signaling pathways (Toll deficient and IMD deficient), which suggests an active microbe/microbe/host interaction. Conclusion: These results indicate that the D. melanogaster systemic infection model is consistent with the study of polymicrobial infection.
    Thematic Areas: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: mariajose.figueras@urv.cat
    Author identifier: 0000-0002-2268-8980
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2076-0817/12/3/405
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Pathogens. 12 (3):
    APA: Robert, Alexandre; Talagrand-Reboul, Emilie; Figueras, Maria-Jose; Ruimy, Raymond; Boyer, Laurent; Lamy, Brigitte; (2023). Drosophila melanogaster Systemic Infection Model to Study Altered Virulence during Polymicrobial Infection by Aeromonas. Pathogens, 12(3), -. DOI: 10.3390/pathogens12030405
    Article's DOI: 10.3390/pathogens12030405
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Immunology and Allergy,Immunology and Microbiology (Miscellaneous),Infectious Diseases,Microbiology,Microbiology (Medical),Molecular Biology
    Pseudomonas-aeruginosa
    Polymicrobial infection
    Opportunistic pathogen
    Microbial interference
    Lessons
    Infection model
    Host
    Drosophila melanogaster
    Disease
    Bacteria
    Aeromonas
    Molecular biology
    Microbiology (medical)
    Microbiology
    Infectious diseases
    Immunology and microbiology (miscellaneous)
    Immunology and microbiology (all)
    Immunology and allergy
    General immunology and microbiology
    Farmacia
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