Articles producció científica> Medicina i Cirurgia

Smoking Suppresses the Therapeutic Potential of Adipose Stem Cells in Crohn’s Disease Patients through Epigenetic Changes

  • Identification data

    Identifier: imarina:9295693
  • Authors:

    Boronat-Toscano, A
    Vañó, I
    Monfort-Ferré, D
    Menacho, M
    Valldosera, G
    Caro, A
    Espina, B
    Mañas, MJ
    Marti, M
    Espin, E
    Saera-Vila, A
    Serena, C
    Cells
    10.3390/cells12071021
    Cells. 12 (7): 1021-
  • Others:

    Author, as appears in the article.: Boronat-Toscano, A; Vañó, I; Monfort-Ferré, D; Menacho, M; Valldosera, G; Caro, A; Espina, B; Mañas, MJ; Marti, M; Espin, E; Saera-Vila, A; Serena, C
    Department: Medicina i Cirurgia
    URV's Author/s: Boronat Toscano, Albert / Menacho Viladot, Margarita / Monfort Ferre, Diandra / Serena Perelló, Carolina
    Keywords: Tobacco Macrophages Inflammatory bowel diseases Immune cells Dna methylation Differentially methylated regions Cigarette Chronic inflammatory disease Cell therapy Adipose tissue tobacco macrophages inflammatory bowel diseases immune cells differentially methylated regions cigarette chronic inflammatory disease cell therapy adipose tissue
    Abstract: Patients with Crohn’s disease (CD) who smoke are known to have a worse prognosis than never-smokers and a higher risk for post-surgical recurrence, whereas patients who quit smoking after surgery have significantly lower post-operative recurrence. The hypothesis was that smoking induces epigenetic changes that impair the capacity of adipose stem cells (ASCs) to suppress the immune system. It was also questioned whether this impairment remains in ex-smokers with CD. ASCs were isolated from non-smokers, smokers and ex-smokers with CD and their interactions with immune cells were studied. The ASCs from both smokers and ex-smokers promoted macrophage polarization to an M1 pro-inflammatory phenotype, were not able to inhibit T- and B-cell proliferation in vitro and enhanced the gene and protein expression of inflammatory markers including interleukin-1b. Genome-wide epigenetic analysis using two different bioinformatic approaches revealed significant changes in the methylation patterns of genes that are critical for wound healing, immune and metabolic response and p53-mediated DNA damage response in ASCs from smokers and ex-smokers with CD. In conclusion, cigarette smoking induces a pro-inflammatory epigenetic signature in ASCs that likely compromises their therapeutic potential.
    Thematic Areas: Medicine (miscellaneous) Cell biology Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: margarita.menacho@urv.cat carolina.serena@urv.cat albert.boronat@estudiants.urv.cat diandra.monfort@estudiants.urv.cat
    Author identifier: 0000-0003-3832-4249
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2073-4409/12/7/1021
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Cells. 12 (7): 1021-
    APA: Boronat-Toscano, A; Vañó, I; Monfort-Ferré, D; Menacho, M; Valldosera, G; Caro, A; Espina, B; Mañas, MJ; Marti, M; Espin, E; Saera-Vila, A; Serena, C (2023). Smoking Suppresses the Therapeutic Potential of Adipose Stem Cells in Crohn’s Disease Patients through Epigenetic Changes. Cells, 12(7), 1021-. DOI: 10.3390/cells12071021
    Article's DOI: 10.3390/cells12071021
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Cell Biology,Medicine (Miscellaneous)
    Tobacco
    Macrophages
    Inflammatory bowel diseases
    Immune cells
    Dna methylation
    Differentially methylated regions
    Cigarette
    Chronic inflammatory disease
    Cell therapy
    Adipose tissue
    tobacco
    macrophages
    inflammatory bowel diseases
    immune cells
    differentially methylated regions
    cigarette
    chronic inflammatory disease
    cell therapy
    adipose tissue
    Medicine (miscellaneous)
    Cell biology
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
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