Articles producció científica> Medicina i Cirurgia

Expression of STING in Women with Morbid Obesity and Nonalcoholic Fatty Liver Disease

  • Identification data

    Identifier: imarina:9296659
    Authors:
    Bertran, LaiaAdalid, LaiaVilaro-Blay, MerceBarrientos-Riosalido, AndreaAguilar, CarmenMartinez, SalomeSabench, Fatimadel Castillo, DanielPorras, Jose AntonioAlibalic, AjlaRichart, CristobalAuguet, Teresa
    Abstract:
    Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic hepatic disease. Although mostly benign, this disease can evolve into nonalcoholic steatohepatitis (NASH). The stimulator of interferon genes (STING) plays an important role in the immune response against stressed cells, but this protein may also be involved in liver lipogenesis and microbiota composition. In this study, the role of STING in NAFLD was evaluated by RT–qPCR to analyze STING mRNA abundance and by immunohistochemical analysis to evaluate protein expression in liver biopsies from a cohort composed of 69 women with morbid obesity classified according to their liver involvement (normal liver, n = 27; simple steatosis (SS), n = 26; NASH, n = 16). The results showed that STING mRNA expression in the liver increases with the occurrence of NAFLD, specifically in the SS stage in which the degree of steatosis is mild or moderate. Protein analysis corroborated these results. Positive correlations were observed among hepatic STING mRNA abundance and gamma-glutamyl transferase and alkaline phosphatase levels, hepatic Toll-like receptor 9 expression and some circulating microbiota-derived bile acids. In conclusion, STING may be involved in the outcome and progression of NAFLD and may be related to hepatic lipid metabolism. However, further studies are needed to confirm these findings.
  • Others:

    Author, as appears in the article.: Bertran, Laia; Adalid, Laia; Vilaro-Blay, Merce; Barrientos-Riosalido, Andrea; Aguilar, Carmen; Martinez, Salome; Sabench, Fatima; del Castillo, Daniel; Porras, Jose Antonio; Alibalic, Ajla; Richart, Cristobal; Auguet, Teresa
    Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques
    URV's Author/s: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Barrientos Riosalido, Andrea / Bertran Ramos, Laia / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima
    Keywords: Stimulator of interferon genes Nonalcoholic fatty liver disease Microbiota Lipogenesis Inflammation stimulator of interferon genes pathway microbiota lipogenesis inflammation cirrhosis
    Abstract: Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic hepatic disease. Although mostly benign, this disease can evolve into nonalcoholic steatohepatitis (NASH). The stimulator of interferon genes (STING) plays an important role in the immune response against stressed cells, but this protein may also be involved in liver lipogenesis and microbiota composition. In this study, the role of STING in NAFLD was evaluated by RT–qPCR to analyze STING mRNA abundance and by immunohistochemical analysis to evaluate protein expression in liver biopsies from a cohort composed of 69 women with morbid obesity classified according to their liver involvement (normal liver, n = 27; simple steatosis (SS), n = 26; NASH, n = 16). The results showed that STING mRNA expression in the liver increases with the occurrence of NAFLD, specifically in the SS stage in which the degree of steatosis is mild or moderate. Protein analysis corroborated these results. Positive correlations were observed among hepatic STING mRNA abundance and gamma-glutamyl transferase and alkaline phosphatase levels, hepatic Toll-like receptor 9 expression and some circulating microbiota-derived bile acids. In conclusion, STING may be involved in the outcome and progression of NAFLD and may be related to hepatic lipid metabolism. However, further studies are needed to confirm these findings.
    Thematic Areas: Molecular biology Medicina ii Farmacia Endocrinology, diabetes and metabolism Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry & molecular biology Biochemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: andrea.barrientos@urv.cat andrea.barrientos@urv.cat joseantonio.porras@urv.cat mariasalome.martinez@urv.cat danieldel.castillo@urv.cat laia.bertranr@estudiants.urv.cat laia.bertranr@estudiants.urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Author identifier: 0000-0001-6418-1822 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-9052-1368 0000-0001-9052-1368 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Record's date: 2024-09-28
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2218-1989/13/4/496
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Metabolites. 13 (4): 496-
    APA: Bertran, Laia; Adalid, Laia; Vilaro-Blay, Merce; Barrientos-Riosalido, Andrea; Aguilar, Carmen; Martinez, Salome; Sabench, Fatima; del Castillo, Danie (2023). Expression of STING in Women with Morbid Obesity and Nonalcoholic Fatty Liver Disease. Metabolites, 13(4), 496-. DOI: 10.3390/metabo13040496
    Article's DOI: 10.3390/metabo13040496
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry,Biochemistry & Molecular Biology,Endocrinology, Diabetes and Metabolism,Molecular Biology
    Stimulator of interferon genes
    Nonalcoholic fatty liver disease
    Microbiota
    Lipogenesis
    Inflammation
    stimulator of interferon genes
    pathway
    microbiota
    lipogenesis
    inflammation
    cirrhosis
    Molecular biology
    Medicina ii
    Farmacia
    Endocrinology, diabetes and metabolism
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
    Biochemistry & molecular biology
    Biochemistry
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