A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

Identifier:  imarina:9297460

Handle: https://hdl.handle.net/20.500.11797/imarina9297460

Authors:  Pla, Luis; Xifre-Perez, Elisabet; Ribes, Angela; Aznar, Elena; Dolores Marcos, M; Dolores Marcos, M; Marsal, Lluis F; Marsal, Lluis F; Martinez-Manez, Ramon; Sancenon, Felix

Abstract:
A nanoporous anodic alumina (NAA)-based sensor system for the detection of Mycoplasma was developed through the implementation of "molecular gates" selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies mu L-1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.