Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

  • Identification data

    Identifier: imarina:9297460
    Handle: https://hdl.handle.net/20.500.11797/imarina9297460
    Authors:
    Pla, LuisXifre-Perez, ElisabetRibes, AngelaAznar, ElenaDolores Marcos, MMarsal, Lluis FMartinez-Manez, RamonSancenon, Felix
    Abstract:
    A nanoporous anodic alumina (NAA)-based sensor system for the detection of Mycoplasma was developed through the implementation of "molecular gates" selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies mu L-1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.

  • Others:

    Author, as appears in the article.: Pla, Luis; Xifre-Perez, Elisabet; Ribes, Angela; Aznar, Elena; Dolores Marcos, M; Marsal, Lluis F; Martinez-Manez, Ramon; Sancenon, Felix
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: Marsal Garví, Luis Francisco / Xifré Pérez, Elisabet
    Keywords: Silica mesoporous nanoparticles Rugate filters Rapid detection Oxide Nanoporous anodic alumina Mycoplasma detection Label-free detection Hard anodization Gated materials Dna Carbon nanotubes Cancer Biosensor mycoplasma detection gated materials dna
    Abstract: A nanoporous anodic alumina (NAA)-based sensor system for the detection of Mycoplasma was developed through the implementation of "molecular gates" selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies mu L-1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.
    Thematic Areas: Química General chemistry Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all)
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: elisabet.xifre@urv.cat lluis.marsal@urv.cat
    Author identifier: 0000-0001-6072-9889 0000-0002-5976-1408
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cplu.201600651
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Chempluschem. 82 (3): 337-341
    APA: Pla, Luis; Xifre-Perez, Elisabet; Ribes, Angela; Aznar, Elena; Dolores Marcos, M; Marsal, Lluis F; Martinez-Manez, Ramon; Sancenon, Felix (2017). A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina. Chempluschem, 82(3), 337-341. DOI: 10.1002/cplu.201600651
    Article's DOI: 10.1002/cplu.201600651
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications

  • Keywords:

    Chemistry (Miscellaneous),Chemistry, Multidisciplinary
    Silica mesoporous nanoparticles
    Rugate filters
    Rapid detection
    Oxide
    Nanoporous anodic alumina
    Mycoplasma detection
    Label-free detection
    Hard anodization
    Gated materials
    Dna
    Carbon nanotubes
    Cancer
    Biosensor
    mycoplasma detection
    gated materials
    dna
    Química
    General chemistry
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)

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