Author, as appears in the article.: Ribes, Angela; Aznar, Elena; Sancenon, Felix; Pardo, Teresa; Marsal, Lluis F; Martinez-Manez, Ramon
Department: Enginyeria Electrònica, Elèctrica i Automàtica
URV's Author/s: Marsal Garví, Luis Francisco / Xifré Pérez, Elisabet
Keywords: Systems Specificity Responsive drug-delivery Platform Oral fluid Mesoporous silica nanoparticles Dna Bioresponsive controlled-release Aptasensor Aptamer-based sensor
Abstract: We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement "molecular gates" for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked by the subsequent hybridisation of a specific cocaine aptamer. The response of the gated material was studied in aqueous solution. In a typical experiment, the support was immersed in hybridisation buffer solution in the absence or presence of cocaine. At certain times, the release of rhodamine B from pore voids was measured by fluorescence spectroscopy. The capped NAA support showed poor cargo delivery, but presence of cocaine in the solution selectively induced rhodamine B release. By this simple procedure a limit of detection as low as 5 x 10(-7) M was calculated for cocaine. The gated NAA was successfully applied to detect cocaine in saliva samples and the possible re-use of the nanostructures was assessed. Based on these results, we believe that NAA could be a suitable support to prepare optical gated probes with a synergic combination of the favourable features of selected gated sensing systems and NAA.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: elisabet.xifre@urv.cat lluis.marsal@urv.cat
Author identifier: 0000-0001-6072-9889 0000-0002-5976-1408
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.nature.com/articles/srep38649
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Scientific Reports. 6 38649-
APA: Ribes, Angela; Aznar, Elena; Sancenon, Felix; Pardo, Teresa; Marsal, Lluis F; Martinez-Manez, Ramon (2016). Molecular gated nanoporous anodic alumina for the detection of cocaine. Scientific Reports, 6(), 38649-. DOI: 10.1038/srep38649
Article's DOI: 10.1038/srep38649
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV