Author, as appears in the article.: Crescenti, Anna; Sola, Rosa; Valls, Rosa M.; Caimari, Antoni; del Bas, Josep M.; Anguera, Anna; Angles, Neus; Arola, Lluis;
Department: Bioquímica i Biotecnologia
URV's Author/s: Arola Ferrer, Luis Maria / Solà Alberich, Rosa Maria / Valls Zamora, Rosa Maria
Keywords: Variants Polymorphism Inhibition Impact Hypomethylation Hypermethylation Genistein Genes Expression Dietary polyphenols
Abstract: DNA methylation regulates gene expression and can be modified by different bioactive compounds in foods, such as polyphenols. Cocoa is a rich source of polyphenols, but its role in DNA methylation is still unknown. The objective was to assess the effect of cocoa consumption on DNA methylation and to determine whether the enzymes involved in the DNA methylation process participate in the mechanisms by which cocoa exerts these effects in humans. The global DNA methylation levels in the peripheral blood were evaluated in 214 volunteers who were pre-hypertensive, stage-1 hypertensive or hypercholesterolemic. The volunteers were divided into two groups: 110 subjects who consumed cocoa (6 g/d) for two weeks and 104 control subjects. In addition, the peripheral blood mononuclear cells (PBMCs) from six subjects were treated with a cocoa extract to analyze the mRNA levels of the DNA methyltransferases (DNMTs), methylenetetrahydrofolate reductase (MTHFR), and methionine synthase reductase (MTRR) genes. Cocoa consumption significantly reduced the DNA methylation levels (2.991 +/- 0.366 vs. 3.909 +/- 0.380, p<0.001). Additionally, we found an association between the cocoa effects on DNA methylation and three polymorphisms located in the MTHFR, MTRR, and DNMT3B genes. Furthermore, in PBMCs, the cocoa extract significantly lowered the mRNA levels of the DNMTs, MTHFR, and MTRR. Our study demonstrates for the first time that the consumption of cocoa decreases the global DNA methylation of peripheral leukocytes in humans with cardiovascular risk factors. In vitro experiments with PBMCs suggest that cocoa may exert this effect partially via the down-regulation of DNMTs, MTHFR and MTRR, which are key genes involved in this epigenetic process.
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licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: lluis.arola@urv.cat rosa.sola@urv.cat rosamaria.valls@urv.cat
Author identifier: 0000-0003-2767-1974 0000-0002-8359-235X 0000-0002-3351-0942
Record's date: 2023-05-20
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0065744
Papper original source: Plos One. 8 (6):
APA: Crescenti, Anna; Sola, Rosa; Valls, Rosa M.; Caimari, Antoni; del Bas, Josep M.; Anguera, Anna; Angles, Neus; Arola, Lluis; (2013). Cocoa Consumption Alters the Global DNA Methylation of Peripheral Leukocytes in Humans with Cardiovascular Disease Risk Factors: A Randomized Controlled Trial. Plos One, 8(6), -. DOI: 10.1371/journal.pone.0065744
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1371/journal.pone.0065744
Entity: Universitat Rovira i Virgili
Journal publication year: 2013
Publication Type: Journal Publications