Articles producció científica> Bioquímica i Biotecnologia

Experimental evolution forcing Oenococcus oeni acid tolerance highlights critical role of the citrate locus

  • Identification data

    Identifier: imarina:9324075
    Authors:
    Julliat, FEicher, CTourti, NGlaser, PCabanel, NCoulon, JFavier, MAlexandre, HReguant, CGuyot, SGrandvalet, C
    Abstract:
    Oenococcus oeni is the main lactic acid bacterium associated with malolactic fermentation (MLF) of wines. MLF plays an important role in determining the final quality of wines. Nevertheless, due to the stressful conditions inherent to wine and especially acidity, MLF may be delayed. This study aimed to explore by adaptive evolution improvements in the acid tolerance of starters but also to gain a better understanding of the mechanisms involved in adaptation toward acidity. Four independent populations of the O. oeni ATCC BAA-1163 strain were propagated (approximately 560 generations) in a temporally varying environment, consisting in a gradual pH decrease from pH 5.3 to pH 2.9. Whole genome sequence comparison of these populations revealed that more than 45% of the substituted mutations occurred in only five loci for the evolved populations. One of these five fixed mutations affects mae, the first gene of the citrate operon. When grown in an acidic medium supplemented with citrate, a significantly higher bacterial biomass was produced with the evolved populations compared to the parental strain. Furthermore, the evolved populations slowed down their citrate consumption at low pH without impacting malolactic performance.
  • Others:

    Author, as appears in the article.: Julliat, F; Eicher, C; Tourti, N; Glaser, P; Cabanel, N; Coulon, J; Favier, M; Alexandre, H; Reguant, C; Guyot, S; Grandvalet, C
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Reguant Miranda, Cristina
    Keywords: Proton motive force Lactic acid bacteria Directed evolution Citrate Biological evolution Acidic environment wine stress-response resistance metabolism malolactic fermentation leuconostoc-oenos lactococcus-lactis identification gene-expression directed evolution citrate biological evolution acidic environment
    Abstract: Oenococcus oeni is the main lactic acid bacterium associated with malolactic fermentation (MLF) of wines. MLF plays an important role in determining the final quality of wines. Nevertheless, due to the stressful conditions inherent to wine and especially acidity, MLF may be delayed. This study aimed to explore by adaptive evolution improvements in the acid tolerance of starters but also to gain a better understanding of the mechanisms involved in adaptation toward acidity. Four independent populations of the O. oeni ATCC BAA-1163 strain were propagated (approximately 560 generations) in a temporally varying environment, consisting in a gradual pH decrease from pH 5.3 to pH 2.9. Whole genome sequence comparison of these populations revealed that more than 45% of the substituted mutations occurred in only five loci for the evolved populations. One of these five fixed mutations affects mae, the first gene of the citrate operon. When grown in an acidic medium supplemented with citrate, a significantly higher bacterial biomass was produced with the evolved populations compared to the parental strain. Furthermore, the evolved populations slowed down their citrate consumption at low pH without impacting malolactic performance.
    Thematic Areas: Saúde coletiva Odontología Molecular biology Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Geociências Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: cristina.reguant@urv.cat
    Author identifier: 0000-0002-5036-1408
    Record's date: 2024-08-03
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/pii/S0923250823000232
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Research In Microbiology. 174 (5):
    APA: Julliat, F; Eicher, C; Tourti, N; Glaser, P; Cabanel, N; Coulon, J; Favier, M; Alexandre, H; Reguant, C; Guyot, S; Grandvalet, C (2023). Experimental evolution forcing Oenococcus oeni acid tolerance highlights critical role of the citrate locus. Research In Microbiology, 174(5), -. DOI: 10.1016/j.resmic.2023.104048
    Article's DOI: 10.1016/j.resmic.2023.104048
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Medicine (Miscellaneous),Microbiology,Molecular Biology
    Proton motive force
    Lactic acid bacteria
    Directed evolution
    Citrate
    Biological evolution
    Acidic environment
    wine
    stress-response
    resistance
    metabolism
    malolactic fermentation
    leuconostoc-oenos
    lactococcus-lactis
    identification
    gene-expression
    directed evolution
    citrate
    biological evolution
    acidic environment
    Saúde coletiva
    Odontología
    Molecular biology
    Microbiology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    Geociências
    Farmacia
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
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