Author, as appears in the article.: Jordá T; Rozès N; Martínez-Pastor MT; Puig S
Department: Bioquímica i Biotecnologia
URV's Author/s: Rozès, Nicolas Andre Louis
Keywords: Yeast Ethanol Ergosterol Erg Cth2 kinase ergosterol erg decay cth2
Abstract: Sterol synthesis is an iron-dependent metabolic pathway in eukaryotes. Consequently, fungal ergosterol biosynthesis (ERG) is down-regulated in response to iron deficiency. In this report, we show that, upon iron limitation or overexpression of the iron-regulated mRNA-binding protein Cth2, the yeast Saccharomyces cerevisiae down-regulates the three initial enzymatic steps of ergosterol synthesis (ERG1, ERG7 and ERG11). Mechanistically, we show that Cth2 protein limits the translation and promotes the decrease in the mRNA levels of these specific ERG genes, which contain consensus Cth2-binding sites defined as AU-rich elements (AREs). Thus, expression of CTH2 leads to the accumulation of initial sterol intermediates, such as squalene, and to the drop of ergosterol levels. Changes in CTH2 expression levels disturb the response of yeast cells to stresses related to membrane integrity such as high ethanol and sorbitol concentrations. Therefore, CTH2 should be considered as a critical regulatory factor of ergosterol biosynthesis during iron deficiency.Copyright © 2023. Published by Elsevier B.V.
Thematic Areas: Structural biology Molecular biology Medicina i Genetics Farmacia Ciências biológicas ii Ciências biológicas i Biotecnología Biophysics Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: nicolasrozes@urv.cat
Author identifier: 0000-0001-9718-3429
Record's date: 2024-08-03
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.sciencedirect.com/science/article/pii/S1874939923000548
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms. 1866 (3): 194959-194959
APA: Jordá T; Rozès N; Martínez-Pastor MT; Puig S (2023). The yeast mRNA-binding protein Cth2 post-transcriptionally modulates ergosterol biosynthesis in response to iron deficiency. Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms, 1866(3), 194959-194959. DOI: 10.1016/j.bbagrm.2023.194959
Article's DOI: 10.1016/j.bbagrm.2023.194959
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV