Author, as appears in the article.: Guardia-Escote, Laia; Biosca-Brull, Judit; Cabre, Maria; Blanco, Jordi; Mladenova-Koleva, Mikaela; Basaure, Pia; Perez-Fernandez, Cristian; Sanchez-Santed, Fernando; Domingo, Jose L; Colomina, Maria Teresa
Department: Psicologia; Ciències Mèdiques Bàsiques; Bioquímica i Biotecnologia
URV's Author/s: BASAURE GARCÍA, PÍA ISABEL / Biosca Brull, Judit / Blanco Pérez, Jordi / Cabré Bargalló, Maria / Colomina Fosch, Maria Teresa / Domingo Roig, José Luis / Guardia Escoté, Laia
Keywords: Mice, transgenic; Mice, inbred c57bl; Mice; Male; Lipidomics; Insecticides; Chlorpyrifos; Central-nervous-system; Brain development; Brain; Apolipoproteins e; Apolipoprotein e4; Apolipoprotein e3; Apoe; Animals; targeted replacement; susceptibility; neurotoxicity; metabolism; lipids; insecticide chlorpyrifos; cholesterol; chlorpyrifos; brain development; apolipoprotein-e; apoe; alzheimers-disease
Abstract: Lipids are a major component of the brain, and are involved in structural and neurodevelopmental processes such as neurogenesis, synaptogenesis and signaling. Apolipoprotein E (apoE) is the main lipoprotein involved in lipid transport in the brain. The apoE isoforms can determine vulnerability to the toxic effects of the pesticide chlorpyrifos (CPF), which can interfere with normal neurodevelopment. We aimed to study the effects of postnatal exposure to CPF and of the APOE genotype on the lipid composition of the brain at early ages. For it, we used apoE3 and apoE4 targeted-replacement (TR) male mice, as well as wild-type C57BL/6. The mice were orally exposed to 1 mg/kg/day of CPF on postnatal days 10-15 and, four hours after the treatment, we obtained samples to assess the cerebral lipid composition. Differences between APOE genotypes were found in the cerebral lipid profile in the postnatal period. ApoE4-TR mice exhibited higher lipid concentrations compared to the other groups in most of the cases. CPF exposure led to a decrease in cholesteryl ester and triglyceride concentrations, while modulating the levels of phosphatidylcholine species based on the apoE isoform. Specifically, CPF treatment decreased the concentration of some species of this lipid (PC30:0, PC31:0, PC32:2, PC36:5, PC40:4 and PC40:5) in C57BL/6 mice exposed to CPF, increased (PC31:0 and PC37:6) in apoE3-TR exposed mice while exposed apoE4-TR mice remained unaltered. These results provide further insights into the lipid composition of the brain at early ages, and how it can be modulated by environmental and genetic factors.© 2023. The Author(s).
Thematic Areas: Toxicology; Saúde coletiva; Química; Psicología; Odontología; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Health, toxicology and mutagenesis; General medicine; Farmacia; Engenharias iii; Engenharias i; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciência de alimentos; Biotecnología; Biodiversidade
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: maria.cabre@urv.cat; judit.biosca@urv.cat; judit.biosca@urv.cat; joseluis.domingo@urv.cat; jordi.blanco@urv.cat; mariateresa.colomina@urv.cat
Record's date: 2025-02-19
Paper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://link.springer.com/article/10.1007/s00204-023-03555-8
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Archives Of Toxicology. 97 (9): 2463-2475
APA: Guardia-Escote, Laia; Biosca-Brull, Judit; Cabre, Maria; Blanco, Jordi; Mladenova-Koleva, Mikaela; Basaure, Pia; Perez-Fernandez, Cristian; Sanchez-Sa (2023). Developmental brain lipidomics is influenced by postnatal chlorpyrifos exposure and APOE genetic background in mice. Archives Of Toxicology, 97(9), 2463-2475. DOI: 10.1007/s00204-023-03555-8
Article's DOI: 10.1007/s00204-023-03555-8
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV