Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Nano-Roughness-Mediated Macrophage Polarization for Desired Host Immune Response

  • Identification data

    Identifier: imarina:9327706
  • Authors:

    Dabare, PRL
    Bachhuka, A
    Quek, JY
    Marsal, LF
    Hayball, J
    Vasilev, K
  • Others:

    Author, as appears in the article.: Dabare, PRL; Bachhuka, A; Quek, JY; Marsal, LF; Hayball, J; Vasilev, K
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: Bachhuka, Akash / Marsal Garví, Luis Francisco
    Keywords: Topography Surface nanotopography Plasma polymerization Nanotopography Modulation Macrophage response Macrophage polarization Interleukin-10 Fusion Foreign body reaction Differentiation Chemistry Body giant-cells Biomaterials Activation
    Abstract: Macrophage polarization is a significant event in the host immune response, which can be modulated by modifying the surface of a biomaterial. Previous studies have demonstrated the modulation of macrophage polarization using different surface features; however, none of these studies reflect the effect of surface properties on unstimulated macrophage polarization for a prolonged period. To better understand the impact of surface features, in this work differentiated THP-1 cells are employed to control macrophage polarization on nano-rough surfaces for a duration of 7 days. Model nano-rough substrates are fabricated by immobilizing gold nanoparticles (AuNPs) of predetermined sizes (16, 38, 68 nm) on a 2-methyl-2-oxazoline thin film, followed by tailoring the outermost surface chemistry. All modified surfaces support high levels of cell adhesion and proliferation. Over time, the expression of pro-inflammatory cytokines decreases, whereas the expression of anti-inflammatory cytokines increases on all modified surfaces. Similarly, pro-inflammatory interleukin (IL)-1 & beta; gene expression is downregulated, and anti-inflammatory IL-10-gene expression is upregulated, regardless of the surface roughness. Analysis of cell morphology reveals that the predominant cell type on the modified surfaces exhibits M2 anti-inflammatory phenotype. Herein, how surface features can modulate macrophage responses over an extended period is highlighted, offering insights for the development of future biomaterial implants.
    Thematic Areas: Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Chemical engineering (miscellaneous) Catalysis
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: akash.bachhuka@urv.cat lluis.marsal@urv.cat
    Author identifier: 0000-0003-1253-8126 0000-0002-5976-1408
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://onlinelibrary.wiley.com/doi/full/10.1002/smsc.202300080
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Small Science. 3 (10):
    APA: Dabare, PRL; Bachhuka, A; Quek, JY; Marsal, LF; Hayball, J; Vasilev, K (2023). Nano-Roughness-Mediated Macrophage Polarization for Desired Host Immune Response. Small Science, 3(10), -. DOI: 10.1002/smsc.202300080
    Article's DOI: 10.1002/smsc.202300080
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Catalysis,Chemical Engineering (Miscellaneous),Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Nanoscience & Nanotechnology
    Topography
    Surface nanotopography
    Plasma polymerization
    Nanotopography
    Modulation
    Macrophage response
    Macrophage polarization
    Interleukin-10
    Fusion
    Foreign body reaction
    Differentiation
    Chemistry
    Body giant-cells
    Biomaterials
    Activation
    Nanoscience & nanotechnology
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Chemical engineering (miscellaneous)
    Catalysis
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