Author, as appears in the article.: Kodr, David; Ortiz, Mayreli; Sykorova, Veronika; Yenice, Cansu Pinar; Lesnikowski, Zbigniew J; O'Sullivan, Ciara K; Hocek, Michal
Department: Enginyeria Química
URV's Author/s: O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli / Yenice, Cansu Pinar
Keywords: Tyrosine Sensors Nucleoside triphosphates Direct electrochemical detection Aromatic-groups
Abstract: The previously reported approach of orthogonal multipotentialredoxcoding of all four DNA bases allowed only analysis of the relativenucleotide composition of short DNA stretches. Here, we present twomethods for normalization of the electrochemical readout to facilitatethe determination of the total nucleotide composition. The first methodis based on the presence or absence of an internal standard of 7-deaza-2 & PRIME;-deoxyguanosinein a DNA primer. The exact composition of the DNA was elucidated upontwo parallel analyses and the subtraction of the electrochemical signalintensities. The second approach took advantage of a 5 & PRIME;-viologenmodified primer, with this fifth orthogonal redox label acting asa reference for signal normalization, thus allowing accurate electrochemicalsequence analysis in a single read. Both approaches were tested usingvarious sequences, and the voltammetric signals obtained were normalizedusing either the internal standard or the reference label and demonstratedto be in perfect agreement with the actual nucleotide composition,highlighting the potential for targeted DNA sequence analysis.
Thematic Areas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: cansupinar.yenice@urv.cat mayreli.ortiz@urv.cat mayreli.ortiz@urv.cat cansupinar.yenice@urv.cat
Author identifier: 0000-0002-9423-0055 0000-0002-9423-0055
Record's date: 2024-10-26
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://pubs.acs.org/doi/10.1021/acs.analchem.3c02023
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Analytical Chemistry. 95 (34): 12586-12589
APA: Kodr, David; Ortiz, Mayreli; Sykorova, Veronika; Yenice, Cansu Pinar; Lesnikowski, Zbigniew J; O'Sullivan, Ciara K; Hocek, Michal (2023). Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition. Analytical Chemistry, 95(34), 12586-12589. DOI: 10.1021/acs.analchem.3c02023
Article's DOI: 10.1021/acs.analchem.3c02023
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV