Author, as appears in the article.: Cumeras, R; Shen, T; Valdiviez, L; Tippins, Z; Haffner, B; Fiehn, O
Department: Enginyeria Electrònica, Elèctrica i Automàtica
URV's Author/s: Cumeras Olmeda, Raquel
Keywords: Vegan Tool Stool Reference material Omnivore Metabolomics Mass spectrometry Diet vegan stool omnivore metabolomics mass spectrometry diet
Abstract: To gain confidence in results of omic-data acquisitions, methods must be benchmarked using validated quality control materials. We report data combining both untargeted and targeted metabolomics assays for the analysis of four new human fecal reference materials developed by the U.S. National Institute of Standards and Technologies (NIST) for metagenomics and metabolomics measurements. These reference grade test materials (RGTM) were established by NIST based on two different diets and two different samples treatments, as follows: firstly, homogenized fecal matter from subjects eating vegan diets, stored and submitted in either lyophilized (RGTM 10162) or aqueous form (RGTM 10171); secondly, homogenized fecal matter from subjects eating omnivore diets, stored and submitted in either lyophilized (RGTM 10172) or aqueous form (RGTM 10173). We used four untargeted metabolomics assays (lipidomics, primary metabolites, biogenic amines and polyphenols) and one targeted assay on bile acids. A total of 3563 compounds were annotated by mass spectrometry, including 353 compounds that were annotated in more than one assay. Almost half of all compounds were annotated using hydrophilic interaction chromatography/accurate mass spectrometry, followed by the lipidomics and the polyphenol assays. In total, 910 metabolites were found in at least 4-fold different levels in fecal matter from vegans versus omnivores, specifically for peptides, amino acids and lipids. In comparison, only 251 compounds showed 4-fold differences between lyophilized and aqueous fecal samples, including DG O-34:0 and methionine sulfoxide. A range of diet-specific metabolites were identified to be significantly different between vegans and omnivores, exemplified by citrinin and C17:0-acylcarnitine for omnivores, and curcumin and lenticin for vegans. Bioactive molecules like acyl alpha-hydroxy-fatty acids (AAHFA) were differentially regulated in vegan versus omnivore fecal materials, highlighting the importance of diet-specific reference materials for dietary biomarker studies.
Thematic Areas: Molecular biology Medicina ii Farmacia Endocrinology, diabetes and metabolism Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: raquel.cumeras@urv.cat
Author identifier: 0000-0003-4663-4247
Record's date: 2024-08-03
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2218-1989/13/8/921
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Metabolites. 13 (8):
APA: Cumeras, R; Shen, T; Valdiviez, L; Tippins, Z; Haffner, B; Fiehn, O (2023). Differences in the Stool Metabolome between Vegans and Omnivores: Analyzing the NIST Stool Reference Material. Metabolites, 13(8), -. DOI: 10.3390/metabo13080921
Article's DOI: 10.3390/metabo13080921
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV