Articles producció científica> Medicina i Cirurgia

Assessing the Utility of Multiplexed Polymerase Chain Reaction in Detecting Microorganisms Causing Infections in Critically ill Patients

  • Identification data

    Identifier: imarina:9330089
  • Authors:

    Garrido, P
    Gabaldó-Barrios, X
    Pujol-Bajador, I
    Fernández, L
    Ballester, F
    Garrido, R
    Cueto, P
    Camps, J
    Vallverdu, I
  • Others:

    Author, as appears in the article.: Garrido, P; Gabaldó-Barrios, X; Pujol-Bajador, I; Fernández, L; Ballester, F; Garrido, R; Cueto, P; Camps, J; Vallverdu, I
    Department: Medicina i Cirurgia
    URV's Author/s: Camps Andreu, Jorge / Pujol Bajador, Isabel
    Keywords: Antimicrobial therapy tests sepsis resistance pneumonia mortality management impact guidelines
    Abstract: Early sepsis diagnosis is crucial for implementing adequate antibiotic therapy and for patient survival. This study investigated whether using multiplexed PCR for detecting microorganisms in critical septic patients affects initial antibiotic treatment and compared it to microbiological culture. It also explored scenarios where PCR is more effective in clinical practice. One hundred nineteen specimens (83 blood and 36 respiratory specimens) belonging to 93 patients were analyzed. Multiplexed PCR determinations were performed using the FA-BCID Panel (bioMérieux) for blood samples and the FA-Pneumo for respiratory samples. The mean turnaround times were 1.7 h for the FA-BCID and 1.5h for the FA-Pneumo. Conversely, they were 96.1 h for blood cultures and 72.3 h for respiratory cultures. FA-BCID showed a mean sensitivity of 97% and specificity of 100%. FA-Pneumo showed a sensitivity of 100% and specificity of 90%. However, the positive predictive value was only 39%. Discrepancies were common in polymicrobial samples. Based on the PCR results, initial empirical treatment should have been changed in 71% of patients with bloodstream infections and 61% with respiratory infections. We conclude that multiplexed PCR improves the response time in identifying germs with a high degree of coincidence for blood cultures and moderate for respiratory cultures. These results highlight the importance of PCR in choosing an appropriate antibiotic therapy.© 2023. The Author(s).
    Thematic Areas: Zootecnia / recursos pesqueiros Química Odontología Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Geociências Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física Applied microbiology and biotechnology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: jorge.camps@urv.cat isabel.pujol@urv.cat
    Author identifier: 0000-0002-3165-3640
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://link.springer.com/article/10.1007/s00284-023-03461-3
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Current Microbiology. 80 (11): 348-348
    APA: Garrido, P; Gabaldó-Barrios, X; Pujol-Bajador, I; Fernández, L; Ballester, F; Garrido, R; Cueto, P; Camps, J; Vallverdu, I (2023). Assessing the Utility of Multiplexed Polymerase Chain Reaction in Detecting Microorganisms Causing Infections in Critically ill Patients. Current Microbiology, 80(11), 348-348. DOI: 10.1007/s00284-023-03461-3
    Article's DOI: 10.1007/s00284-023-03461-3
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Applied Microbiology and Biotechnology,Medicine (Miscellaneous),Microbiology
    Antimicrobial therapy
    tests
    sepsis
    resistance
    pneumonia
    mortality
    management
    impact
    guidelines
    Zootecnia / recursos pesqueiros
    Química
    Odontología
    Microbiology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
    Astronomia / física
    Applied microbiology and biotechnology
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