Articles producció científica> Ciències Mèdiques Bàsiques

Comprehensive profiling and semi-quantification of exogenous chemicals in human urine using HRMS-based strategies

  • Identification data

    Identifier: imarina:9331785
  • Authors:

    Gutiérrez-Martín D
    Restrepo-Montes E
    Golovko O
    López-Serna R
    Aalizadeh R
    Thomaidis NS
    Marquès M
    Gago-Ferrero P
    Gil-Solsona R
    Analytical And Bioanalytical Chemistry
    10.1007/s00216-023-04998-9
    Analytical And Bioanalytical Chemistry. 415 (29-30): 7297-
  • Others:

    Author, as appears in the article.: Gutiérrez-Martín D; Restrepo-Montes E; Golovko O; López-Serna R; Aalizadeh R; Thomaidis NS; Marquès M; Gago-Ferrero P; Gil-Solsona R
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Marquès Bueno, Montserrat
    Keywords: Non-target Method validation Human biomonitoring (hbm) High-resolution mass spectrometry (hrms) Glucuronides Deconjugation
    Abstract: Chemicals infiltrate our daily experiences through multiple exposure pathways. Human biomonitoring (HBM) is routinely used to comprehensively understand these chemical interactions. Historically, HBM depended on targeted screening methods limited to a relatively small set of chemicals with triple quadrupole instruments typically. However, recent advances in high-resolution mass spectrometry (HRMS) have facilitated the use of broad-scope target, suspect, and non-target strategies, enhancing chemical exposome characterization within acceptable detection limits. Despite these advancements, establishing robust and efficient sample treatment protocols is still essential for trustworthy broad-range chemical analysis. This study sought to validate a methodology leveraging HRMS-based strategies for accurate profiling of exogenous chemicals and related metabolites in urine samples. We evaluated five extraction protocols, each encompassing various chemical classes, such as pharmaceuticals, plastic additives, personal care products, and pesticides, in terms of their extraction recoveries, linearity, matrix effect, sensitivity, and reproducibility. The most effective protocol was extensively validated and subsequently applied to 10 real human urine samples using wide-scope target analysis encompassing over 2000 chemicals. We successfully identified and semi-quantified a total of 36 chemicals using an ionization efficiency-based model, affirming the methodology's robust performance. Notably, our results dismissed the need for a deconjugation step, a typically labor-intensive and time-consuming process.© 2023. The Author(s).
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Educação física Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Biochemistry Biochemical research methods Astronomia / física Analytical chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: montserrat.marques@urv.cat
    Author identifier: 0000-0001-6302-8578
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://link.springer.com/article/10.1007/s00216-023-04998-9#citeas
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Analytical And Bioanalytical Chemistry. 415 (29-30): 7297-
    APA: Gutiérrez-Martín D; Restrepo-Montes E; Golovko O; López-Serna R; Aalizadeh R; Thomaidis NS; Marquès M; Gago-Ferrero P; Gil-Solsona R (2023). Comprehensive profiling and semi-quantification of exogenous chemicals in human urine using HRMS-based strategies. Analytical And Bioanalytical Chemistry, 415(29-30), 7297-. DOI: 10.1007/s00216-023-04998-9
    Article's DOI: 10.1007/s00216-023-04998-9
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Biochemical Research Methods,Biochemistry,Chemistry, Analytical
    Non-target
    Method validation
    Human biomonitoring (hbm)
    High-resolution mass spectrometry (hrms)
    Glucuronides
    Deconjugation
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Educação física
    Educação
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Biochemistry
    Biochemical research methods
    Astronomia / física
    Analytical chemistry
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