Author, as appears in the article.: Gutiérrez-Martín D; Restrepo-Montes E; Golovko O; López-Serna R; Aalizadeh R; Thomaidis NS; Marquès M; Gago-Ferrero P; Gil-Solsona R
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Marquès Bueno, Montserrat
Keywords: Non-target Method validation Human biomonitoring (hbm) High-resolution mass spectrometry (hrms) Glucuronides Deconjugation
Abstract: Chemicals infiltrate our daily experiences through multiple exposure pathways. Human biomonitoring (HBM) is routinely used to comprehensively understand these chemical interactions. Historically, HBM depended on targeted screening methods limited to a relatively small set of chemicals with triple quadrupole instruments typically. However, recent advances in high-resolution mass spectrometry (HRMS) have facilitated the use of broad-scope target, suspect, and non-target strategies, enhancing chemical exposome characterization within acceptable detection limits. Despite these advancements, establishing robust and efficient sample treatment protocols is still essential for trustworthy broad-range chemical analysis. This study sought to validate a methodology leveraging HRMS-based strategies for accurate profiling of exogenous chemicals and related metabolites in urine samples. We evaluated five extraction protocols, each encompassing various chemical classes, such as pharmaceuticals, plastic additives, personal care products, and pesticides, in terms of their extraction recoveries, linearity, matrix effect, sensitivity, and reproducibility. The most effective protocol was extensively validated and subsequently applied to 10 real human urine samples using wide-scope target analysis encompassing over 2000 chemicals. We successfully identified and semi-quantified a total of 36 chemicals using an ionization efficiency-based model, affirming the methodology's robust performance. Notably, our results dismissed the need for a deconjugation step, a typically labor-intensive and time-consuming process.© 2023. The Author(s).
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Educação física Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Biochemistry Biochemical research methods Astronomia / física Analytical chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: montserrat.marques@urv.cat
Author identifier: 0000-0001-6302-8578
Record's date: 2024-07-20
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://link.springer.com/article/10.1007/s00216-023-04998-9#citeas
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Analytical And Bioanalytical Chemistry. 415 (29-30): 7297-
APA: Gutiérrez-Martín D; Restrepo-Montes E; Golovko O; López-Serna R; Aalizadeh R; Thomaidis NS; Marquès M; Gago-Ferrero P; Gil-Solsona R (2023). Comprehensive profiling and semi-quantification of exogenous chemicals in human urine using HRMS-based strategies. Analytical And Bioanalytical Chemistry, 415(29-30), 7297-. DOI: 10.1007/s00216-023-04998-9
Article's DOI: 10.1007/s00216-023-04998-9
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV