Author, as appears in the article.: Moreno, F; Mendez, L; Raner, A; Miralles-Perez, B; Romeu, M; Ramos-Romero, S; Torres, JL; Medina, I
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Miralles Pérez, Bernat / Romeu Ferran, Marta
Keywords: Protein carbonylation Prediabetes Oxidative stress Oxidative damage Omega-3 fish oil Marine natural antioxidants High-fat and high-sucrose diet Cerebellum purkinje protein carbonylation prediabetes oxidative stress marine natural antioxidants high-fat and high-sucrose diet cerebellum brain
Abstract: The regular intake of diets high in saturated fat and sugars increases oxidative stress and has been linked to cognitive decline and premature brain aging. The cerebellum is highly vulnerable to oxidative stress and thus, obesogenic diets might be particularly detrimental to this tissue. However, the precise molecular mechanisms behind obesity-related brain damage are still not clear. Since protein carbonylation, a biomarker of oxidative stress, influences protein functions and is involved in metabolic control, the current investigation addressed the effect of long-term high-fat and high-sucrose diet intake on the cerebellum of Sprague-Dawley rats by deciphering the changes caused in the carbonylated proteome. The antioxidant effects of fish oil supplementation on cerebellar carbonylated proteins were also investigated. Lipid peroxidation products and carbonylated proteins were identified and quantified using immunoassays and 2D-LC-MS/MS in the cerebellum. After 21 weeks of nutritional intervention, the obesogenic diet selectively increased carbonylation of the proteins that participate in ATP homeostasis and glutamate metabolism in the cerebellum. Moreover, the data demonstrated that fish oil supplementation restrained carbonylation of the main protein targets oxidatively damaged by the obesogenic diet, and additionally protected against carbonylation of several other proteins involved in amino acid biosynthesis and neurotransmission. Therefore, dietary interventions with fish oils could help the cerebellum to be more resilient to oxidative damage. The results could shed some light on the effect of high-fat and high-sucrose diets on redox homeostasis in the cerebellum and boost the development of antioxidant-based nutritional interventions to improve cerebellum health.
Thematic Areas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: marta.romeu@urv.cat bernat.miralles@urv.cat
Author identifier: 0000-0002-2131-1858 0000-0003-1294-7069
Record's date: 2024-02-17
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2076-3921/13/1/103
Papper original source: Antioxidants. 13 (1): 13010103-
APA: Moreno, F; Mendez, L; Raner, A; Miralles-Perez, B; Romeu, M; Ramos-Romero, S; Torres, JL; Medina, I (2024). Dietary Marine Oils Selectively Decrease Obesogenic Diet-Derived Carbonylation in Proteins Involved in ATP Homeostasis and Glutamate Metabolism in the Rat Cerebellum. Antioxidants, 13(1), 13010103-. DOI: 10.3390/antiox13010103
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.3390/antiox13010103
Entity: Universitat Rovira i Virgili
Journal publication year: 2024
Publication Type: Journal Publications