Author, as appears in the article.: Coral, David; Attard, Matthew; Pedrol, Eric; Sole, Rosa Maria; Diaz, Francesc; Aguilo, Magdalena; Mateos, Xavier
Department: Química Física i Inorgànica
URV's Author/s: Aguiló Díaz, Magdalena / Díaz González, Francisco Manuel / Mateos Ferré, Xavier / Solé Cartañà, Rosa Maria
Abstract: Body fluids can potentially indicate the presence of non-small cancer cells. Studying these fluids is an emerging field that could be crucial for cancer detection and monitoring treatment effectiveness. Meanwhile, the examination of fluids on a microscopic level is part of the field of microfluidics. This study focuses on the development of a total analysis system that consists of various interconnected structures that are designed to mix, classify, concentrate, and isolate particles in fluids that mimic the behavior of cancer and normal cells. Using the COMSOL Multiphysics software, the device's performance was optimized to use a pressure input of 35 kPa for water or serum and 29.4 kPa for a mixture of liquid and serum samples, which are the optimal pressure inputs. The numerical models were validated by experiments using two types of polystyrene particles, with diameters of 5 and 20 mu m. Moreover, the developed system was applied to monitor the behavior of red blood cells. The microfluidic chip is capable of addressing several challenges through visual detections, including mixing tests of two fluids with similar densities, proper particle size classification using Dean flow fractionation, and single-step recovery of large, labeled particles. Finally, the collected particles were examined using an environmental scanning electron microscope to determine their size, and the results demonstrated that successful size separation was achieved, with particles around 20 mu m completely separated from the smaller ones.
Thematic Areas: Engineering, biomedical Biophysics Biomedical engineering Biomaterials Bioengineering
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: f.diaz@urv.cat magdalena.aguilo@urv.cat rosam.sole@urv.cat xavier.mateos@urv.cat
Author identifier: 0000-0003-4581-4967 0000-0001-6130-9579 0000-0002-5769-4141 0000-0003-1940-1990
Record's date: 2024-08-03
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://pubs.aip.org/aip/apb/article/8/2/026101/3283008/Computational-and-experimental-microfluidics-Total
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Apl Bioengineering. 8 (2): 026101-
APA: Coral, David; Attard, Matthew; Pedrol, Eric; Sole, Rosa Maria; Diaz, Francesc; Aguilo, Magdalena; Mateos, Xavier (2024). Computational and experimental microfluidics: Total analysis system for mixing, sorting, and concentrating particles and cells. Apl Bioengineering, 8(2), 026101-. DOI: 10.1063/5.0158648
Article's DOI: 10.1063/5.0158648
Entity: Universitat Rovira i Virgili
Journal publication year: 2024
Publication Type: Journal Publications