Articles producció científica> Química Física i Inorgànica

Computational and experimental microfluidics: Total analysis system for mixing, sorting, and concentrating particles and cells

  • Identification data

    Identifier: imarina:9366240
    Authors:
    Coral, DavidAttard, MatthewPedrol, EricSole, Rosa MariaDiaz, FrancescAguilo, MagdalenaMateos, Xavier
    Abstract:
    Body fluids can potentially indicate the presence of non-small cancer cells. Studying these fluids is an emerging field that could be crucial for cancer detection and monitoring treatment effectiveness. Meanwhile, the examination of fluids on a microscopic level is part of the field of microfluidics. This study focuses on the development of a total analysis system that consists of various interconnected structures that are designed to mix, classify, concentrate, and isolate particles in fluids that mimic the behavior of cancer and normal cells. Using the COMSOL Multiphysics software, the device's performance was optimized to use a pressure input of 35 kPa for water or serum and 29.4 kPa for a mixture of liquid and serum samples, which are the optimal pressure inputs. The numerical models were validated by experiments using two types of polystyrene particles, with diameters of 5 and 20 mu m. Moreover, the developed system was applied to monitor the behavior of red blood cells. The microfluidic chip is capable of addressing several challenges through visual detections, including mixing tests of two fluids with similar densities, proper particle size classification using Dean flow fractionation, and single-step recovery of large, labeled particles. Finally, the collected particles were examined using an environmental scanning electron microscope to determine their size, and the results demonstrated that successful size separation was achieved, with particles around 20 mu m completely separated from the smaller ones.
  • Others:

    Author, as appears in the article.: Coral, David; Attard, Matthew; Pedrol, Eric; Sole, Rosa Maria; Diaz, Francesc; Aguilo, Magdalena; Mateos, Xavier
    Department: Química Física i Inorgànica
    URV's Author/s: Aguiló Díaz, Magdalena / Díaz González, Francisco Manuel / Mateos Ferré, Xavier / Solé Cartañà, Rosa Maria
    Abstract: Body fluids can potentially indicate the presence of non-small cancer cells. Studying these fluids is an emerging field that could be crucial for cancer detection and monitoring treatment effectiveness. Meanwhile, the examination of fluids on a microscopic level is part of the field of microfluidics. This study focuses on the development of a total analysis system that consists of various interconnected structures that are designed to mix, classify, concentrate, and isolate particles in fluids that mimic the behavior of cancer and normal cells. Using the COMSOL Multiphysics software, the device's performance was optimized to use a pressure input of 35 kPa for water or serum and 29.4 kPa for a mixture of liquid and serum samples, which are the optimal pressure inputs. The numerical models were validated by experiments using two types of polystyrene particles, with diameters of 5 and 20 mu m. Moreover, the developed system was applied to monitor the behavior of red blood cells. The microfluidic chip is capable of addressing several challenges through visual detections, including mixing tests of two fluids with similar densities, proper particle size classification using Dean flow fractionation, and single-step recovery of large, labeled particles. Finally, the collected particles were examined using an environmental scanning electron microscope to determine their size, and the results demonstrated that successful size separation was achieved, with particles around 20 mu m completely separated from the smaller ones.
    Thematic Areas: Engineering, biomedical Biophysics Biomedical engineering Biomaterials Bioengineering
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: f.diaz@urv.cat magdalena.aguilo@urv.cat rosam.sole@urv.cat xavier.mateos@urv.cat
    Author identifier: 0000-0003-4581-4967 0000-0001-6130-9579 0000-0002-5769-4141 0000-0003-1940-1990
    Record's date: 2024-08-03
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://pubs.aip.org/aip/apb/article/8/2/026101/3283008/Computational-and-experimental-microfluidics-Total
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Apl Bioengineering. 8 (2): 026101-
    APA: Coral, David; Attard, Matthew; Pedrol, Eric; Sole, Rosa Maria; Diaz, Francesc; Aguilo, Magdalena; Mateos, Xavier (2024). Computational and experimental microfluidics: Total analysis system for mixing, sorting, and concentrating particles and cells. Apl Bioengineering, 8(2), 026101-. DOI: 10.1063/5.0158648
    Article's DOI: 10.1063/5.0158648
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2024
    Publication Type: Journal Publications
  • Keywords:

    Bioengineering,Biomaterials,Biomedical Engineering,Biophysics,Engineering, Biomedical
    Engineering, biomedical
    Biophysics
    Biomedical engineering
    Biomaterials
    Bioengineering
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