Author, as appears in the article.: Ribas-Maynou J; Novo S; Torres M; Salas-Huetos A; Rovira S; Antich M; Yeste M
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Salas Huetos, Albert
Keywords: Dna Double donation Embryonic development Female Fertilization in vitro Humans Icsi Infertility Male Male-factor Oocyte donors Oocytes Pregnancy Pregnancy rate Semen Sperm Sperm injections, intracytoplasmic Spermatozoa Tissue donors
Abstract: Based on the inconsistent literature published thus far involving infertile patients, whether intracytoplasmic sperm injection (ICSI) allows overcoming total fertilization failure due to sperm DNA fragmentation is still unclear. Related to this, female factors, which may have a significant impact on assisted reproduction outcomes, can mask male infertility. In this scenario, evaluating ICSI outcomes following cycles using healthy donor gametes could shed light on this realm, as it would avoid the influence of (un)known confounding factors present in infertile individuals. The present work, therefore, aimed to address whether single- and double-stranded sperm DNA fragmentation leads to impaired ICSI outcomes in double gamete donation cycles. The study also compared these double-gamete donation cycles to cycles in which only sperm were donated and oocytes were obtained from infertile patients. Two cohorts were included: (a) the Donor-Donor (DD) cohort, which included 27 semen donor samples used in 49 ICSI cycles with young healthy oocyte donors; and (b) the Donor-Infertile (DI) cohort, which involved 34 semen donor samples used in 57 ICSI cycles with oocytes from patients. Single- and double-stranded sperm DNA breaks were determined with alkaline and neutral Comet assays, respectively; ICSI was conducted following standard protocols and embryos were monitored through time-lapse microscopy. In the DD cohort, the percentage of sperm with high overall DNA damage correlated with fertilization rates (Rs = - 0.666; P < 0.001) and with the percentage of blastocysts per injected oocyte (Rs = - 0.414; P = 0.040). In addition, sperm DNA damage delayed the first embryo division (Rs = 0.421; P = 0.036), and development from the 8-cell to the morula stage (Rs = 0.424; P = 0.034). In contrast, double-stranded DNA breaks had no effect in this cohort. As far as the DI cohort is concerned, while overall sperm DNA damage was not found to be correlated to fertilization or blastocyst rates, pronuclei formation following ICSI was delayed when the incidence of double-stranded DNA breaks was high (Rs = 0.485; P = 0.005). In conclusion, this study, which is the first involving double donation cycles (i.e., a donor-donor cohort), supports that sperm DNA damage has a detrimental impact on fertilization rates after ICSI, and delays embryo development. Moreover, the use of oocytes from infertile individuals is suggested to hide the male-factor effect.
Thematic Areas: Agricultural and biological sciences (all) Agricultural and biological sciences (miscellaneous) Biochemistry, genetics and molecular biology (all) Biochemistry, genetics and molecular biology (miscellaneous) Biodiversidade Biology Biotecnología Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Economia Educação física Farmacia General agricultural and biological sciences General biochemistry,genetics and molecular biology General medicine Interdisciplinar Materiais Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (all) Medicine (miscellaneous) Nutrição Química Zootecnia / recursos pesqueiros
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: albert.salas@urv.cat
Author identifier: 0000-0001-5914-6862
Record's date: 2024-07-20
Papper version: info:eu-repo/semantics/publishedVersion
Papper original source: Biological Research. 55 (1): 41-
APA: Ribas-Maynou J; Novo S; Torres M; Salas-Huetos A; Rovira S; Antich M; Yeste M (2022). Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used.. Biological Research, 55(1), 41-. DOI: 10.1186/s40659-022-00409-y
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV