Articles producció científica> Bioquímica i Biotecnologia

A serum metabolic biomarker panel for early rheumatoid arthritis

  • Identification data

    Identifier: imarina:9384822
    Authors:
    Rodriguez-Muguruza, SamanthaAltuna-Coy, AntonioArreaza-Gil, VeronicaMendieta-Homs, MarinaCastro-Oreiro, SoniaPoveda-Elices, Maria Josedel Castillo-Pinol, NuriaFontova-Garrofe, RamonChacon, Matilde R
    Abstract:
    ObjectiveThere is an urgent need for novel biomarkers to improve the early diagnosis of rheumatoid arthritis (ERA). Current serum biomarkers used in the management of ERA, including rheumatoid factor and anti-cyclic citrullinated peptide (ACPA), show limited specificity and sensitivity. Here, we used metabolomics to uncover new serum biomarkers of ERA.MethodsWe applied an untargeted metabolomics approach including gas chromatography time-of-flight mass spectrometry in serum samples from an ERA cohort (n=32) and healthy controls (n=19). Metabolite set enrichment analysis was performed to explore potentially important biological pathways. Partial least squares discriminant analysis and variable importance in projection analysis were performed to construct an ERA biomarker panel.ResultsSignificant differences in the content of 11/81 serum metabolites were identified in patients with ERA. Receiver operating characteristic (ROC) analysis showed that a panel of only three metabolites (glyceric acid, lactic acid, and 3-hydroxisovaleric acid) could correctly classify 96.7% of patients with ERA, with an area under the ROC curve of 0.963 and with 94.4% specificity and 93.5% sensitivity, outperforming ACPA-based diagnosis by 2.9% and, thus, improving the preclinical detection of ERA. Aminoacyl-tRNA biosynthesis and serine, glycine, and phenylalanine metabolism were the most significant dysregulated pathways in patients with ERA.ConclusionA metabolomics serum-based biomarker panel composed of glyceric acid, lactic acid, and 3-hydroxisovaleric acid offers potential for the early clinical diagnosis of RA.
  • Others:

    Author, as appears in the article.: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del Castillo-Pinol, Nuria; Fontova-Garrofe, Ramon; Chacon, Matilde R
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Arreaza Gil, Verónica
    Keywords: Arthritis, rheumatoid Biomarkers Biomarkers 2 Diagnostic Early rheumatoid arthritis Glyceric acid Glyceric acids Humans Lactic acid Metabolomics Serum
    Abstract: ObjectiveThere is an urgent need for novel biomarkers to improve the early diagnosis of rheumatoid arthritis (ERA). Current serum biomarkers used in the management of ERA, including rheumatoid factor and anti-cyclic citrullinated peptide (ACPA), show limited specificity and sensitivity. Here, we used metabolomics to uncover new serum biomarkers of ERA.MethodsWe applied an untargeted metabolomics approach including gas chromatography time-of-flight mass spectrometry in serum samples from an ERA cohort (n=32) and healthy controls (n=19). Metabolite set enrichment analysis was performed to explore potentially important biological pathways. Partial least squares discriminant analysis and variable importance in projection analysis were performed to construct an ERA biomarker panel.ResultsSignificant differences in the content of 11/81 serum metabolites were identified in patients with ERA. Receiver operating characteristic (ROC) analysis showed that a panel of only three metabolites (glyceric acid, lactic acid, and 3-hydroxisovaleric acid) could correctly classify 96.7% of patients with ERA, with an area under the ROC curve of 0.963 and with 94.4% specificity and 93.5% sensitivity, outperforming ACPA-based diagnosis by 2.9% and, thus, improving the preclinical detection of ERA. Aminoacyl-tRNA biosynthesis and serine, glycine, and phenylalanine metabolism were the most significant dysregulated pathways in patients with ERA.ConclusionA metabolomics serum-based biomarker panel composed of glyceric acid, lactic acid, and 3-hydroxisovaleric acid offers potential for the early clinical diagnosis of RA.
    Thematic Areas: Biodiversidade Biotecnología Ciência de alimentos Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Engenharias ii Engenharias iii Farmacia Immunology Immunology and allergy Interdisciplinar Medicina i Medicina ii Medicina iii Medicina veterinaria Nutrição Odontología Química Saúde coletiva Zootecnia / recursos pesqueiros
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: veronica.arreaza@urv.cat veronica.arreaza@urv.cat veronica.arreaza@urv.cat
    Author identifier: 0000-0002-8301-2095 0000-0002-8301-2095 0000-0002-8301-2095
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1253913/full
    Papper original source: Frontiers In Immunology. 14 1253913-
    APA: Rodriguez-Muguruza, Samantha; Altuna-Coy, Antonio; Arreaza-Gil, Veronica; Mendieta-Homs, Marina; Castro-Oreiro, Sonia; Poveda-Elices, Maria Jose; del (2023). A serum metabolic biomarker panel for early rheumatoid arthritis. Frontiers In Immunology, 14(), 1253913-. DOI: 10.3389/fimmu.2023.1253913
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.3389/fimmu.2023.1253913
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Immunology,Immunology and Allergy
    Arthritis, rheumatoid
    Biomarkers
    Biomarkers 2
    Diagnostic
    Early rheumatoid arthritis
    Glyceric acid
    Glyceric acids
    Humans
    Lactic acid
    Metabolomics
    Serum
    Biodiversidade
    Biotecnología
    Ciência de alimentos
    Ciências biológicas i
    Ciências biológicas ii
    Ciências biológicas iii
    Engenharias ii
    Engenharias iii
    Farmacia
    Immunology
    Immunology and allergy
    Interdisciplinar
    Medicina i
    Medicina ii
    Medicina iii
    Medicina veterinaria
    Nutrição
    Odontología
    Química
    Saúde coletiva
    Zootecnia / recursos pesqueiros
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