Articles producció científica> Psicologia

ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa

  • Identification data

    Identifier: imarina:9385426
    Authors:
    Dordas-Perpinya, MartaSergeant, NicolasYanez-Ortiz, IvanMevel, VincentCatalan, JaimeBruyas, Jean-FrancoisBriand-Amirat, LamiaMiro, Jordi
    Abstract:
    ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on the presence of proAKAP4 in donkey sperm, elucidating its localization, molecular characteristics, and its correlation with motility descriptors and mitochondrial membrane potential. Twelve ejaculates from Catalan donkeys were analyzed in this study. The initial steps involved proAKAP4 sequencing and detection through Western blotting and immunofluorescence. Post-thaw assessments were conducted at 0, 1, and 3 h, encompassing proAKAP4 levels, sperm motility analyzed via Computer-Assisted Sperm Analysis (CASA), and mitochondrial membrane potential determined by flow cytometry using the JC-1 stain. The findings reveal that proAKAP4 in donkeys exhibits a characteristic localization at the principal piece of the flagellum, consistent with observations in other mammals. The molecular weight of proAKAP4 is determined to be 100 kDa. Significantly, a positive correlation (p
  • Others:

    Author, as appears in the article.: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi
    Department: Psicologia
    URV's Author/s: Miró Martínez, Jordi
    Keywords: Akap4 Anchor protein Boa Cryopreservation Donkey Mitochondrial activit Motility Proakap4 Semen Sperm Spermatozoa
    Abstract: ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on the presence of proAKAP4 in donkey sperm, elucidating its localization, molecular characteristics, and its correlation with motility descriptors and mitochondrial membrane potential. Twelve ejaculates from Catalan donkeys were analyzed in this study. The initial steps involved proAKAP4 sequencing and detection through Western blotting and immunofluorescence. Post-thaw assessments were conducted at 0, 1, and 3 h, encompassing proAKAP4 levels, sperm motility analyzed via Computer-Assisted Sperm Analysis (CASA), and mitochondrial membrane potential determined by flow cytometry using the JC-1 stain. The findings reveal that proAKAP4 in donkeys exhibits a characteristic localization at the principal piece of the flagellum, consistent with observations in other mammals. The molecular weight of proAKAP4 is determined to be 100 kDa. Significantly, a positive correlation (p
    Thematic Areas: Agriculture, dairy & animal science Animal science and zoology Biodiversidade Biotecnología Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Economia Endocrinology Farmacia Food animals Geografía Interdisciplinar Materiais Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (miscellaneous) Nutrição Odontología Química Reproductive biology Veterinary sciences Zootecnia / recursos pesqueiros
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: jordi.miro@urv.cat
    Author identifier: 0000-0002-1998-6653
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/pii/S0378432024000186?via%3Dihub
    Papper original source: Animal Reproduction Science. 262 107427-
    APA: Dordas-Perpinya, Marta; Sergeant, Nicolas; Yanez-Ortiz, Ivan; Mevel, Vincent; Catalan, Jaime; Bruyas, Jean-Francois; Briand-Amirat, Lamia; Miro, Jordi (2024). ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa. Animal Reproduction Science, 262(), 107427-. DOI: 10.1016/j.anireprosci.2024.107427
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.anireprosci.2024.107427
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2024
    Publication Type: Journal Publications
  • Keywords:

    Agriculture, Dairy & Animal Science,Animal Science and Zoology,Endocrinology,Food Animals,Medicine (Miscellaneous),Reproductive Biology,Veterinary Sciences
    Akap4
    Anchor protein
    Boa
    Cryopreservation
    Donkey
    Mitochondrial activit
    Motility
    Proakap4
    Semen
    Sperm
    Spermatozoa
    Agriculture, dairy & animal science
    Animal science and zoology
    Biodiversidade
    Biotecnología
    Ciências agrárias i
    Ciências ambientais
    Ciências biológicas i
    Ciências biológicas ii
    Ciências biológicas iii
    Economia
    Endocrinology
    Farmacia
    Food animals
    Geografía
    Interdisciplinar
    Materiais
    Medicina i
    Medicina ii
    Medicina iii
    Medicina veterinaria
    Medicine (miscellaneous)
    Nutrição
    Odontología
    Química
    Reproductive biology
    Veterinary sciences
    Zootecnia / recursos pesqueiros
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