Articles producció científicaMedicina i Cirurgia

Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice

  • Identification data

    Identifier:  imarina:9436265
    Handle: https://hdl.handle.net/20.500.11797/imarina9436265
    Authors:  Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia
    Abstract:
    White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and processing mature adipocytes, alongside the cultivation of WAT explants from humans and mice. We describe steps for WAT retrieval, culturing of WAT explants, WAT digestion, and adipocytes separation. We then detail procedures for culturing isolated mature adipocytes. For complete details on the use and execution of this protocol, please refer to Villanueva-Carmona et al. (2023).1

  • Others:

    Link to the original source: https://www.sciencedirect.com/science/article/pii/S2666166723006603
    APA: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia (2023). Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice. Star Protocols, 4(4), 102693-. DOI: 10.1016/j.xpro.2023.102693
    Paper original source: Star Protocols. 4 (4): 102693-
    Article's DOI: 10.1016/j.xpro.2023.102693
    Journal publication year: 2023
    Entity: Universitat Rovira i Virgili
    Paper version: info:eu-repo/semantics/publishedVersion
    Record's date: 2025-02-01
    URV's Author/s: Maymo Masip, Elsa / Vendrell Ortega, Juan José
    Department: Bioquímica i Biotecnologia, Medicina i Cirurgia
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Publication Type: Journal Publications
    Author, as appears in the article.: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Thematic Areas: Biochemical research methods, Biochemistry, genetics and molecular biology (all), Biochemistry, genetics and molecular biology (miscellaneous), Immunology and microbiology (all), Immunology and microbiology (miscellaneous), Neuroscience (all), Neuroscience (miscellaneous)
    Author's mail: jvortega@iispv.cat, elsa.maymo@urv.cat

  • Keywords:

    Adipocytes
    Adipose tissue
    white
    Animals
    Cell culture
    Cell isolation
    Humans
    Metabolism
    Mice
    Model organisms
    Biochemical Research Methods
    Biochemistry
    Genetics and Molecular Biology (Miscellaneous)
    Immunology and Microbiology (Miscellaneous)
    Neuroscience (Miscellaneous)
    genetics and molecular biology (all)
    Immunology and microbiology (all)
    Neuroscience (all)

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