Articles producció científica> Medicina i Cirurgia

Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice

  • Identification data

    Identifier: imarina:9436265
    Handle: https://hdl.handle.net/20.500.11797/imarina9436265
    Authors:
    Villanueva-Carmona, TeresaCedo, LidiaNunez-Roa, CatalinaMaymo-Masip, ElsaVendrell, JoanFernandez-Veledo, Sonia
    Abstract:
    White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and processing mature adipocytes, alongside the cultivation of WAT explants from humans and mice. We describe steps for WAT retrieval, culturing of WAT explants, WAT digestion, and adipocytes separation. We then detail procedures for culturing isolated mature adipocytes. For complete details on the use and execution of this protocol, please refer to Villanueva-Carmona et al. (2023).1

  • Others:

    Author, as appears in the article.: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia
    Department: Bioquímica i Biotecnologia Medicina i Cirurgia
    URV's Author/s: Maymo Masip, Elsa / Vendrell Ortega, Juan José
    Keywords: Adipocytes Adipose tissue, white Animals Cell culture Cell isolation Humans Metabolism Mice Model organisms
    Abstract: White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and processing mature adipocytes, alongside the cultivation of WAT explants from humans and mice. We describe steps for WAT retrieval, culturing of WAT explants, WAT digestion, and adipocytes separation. We then detail procedures for culturing isolated mature adipocytes. For complete details on the use and execution of this protocol, please refer to Villanueva-Carmona et al. (2023).1
    Thematic Areas: Biochemical research methods Biochemistry, genetics and molecular biology (all) Biochemistry, genetics and molecular biology (miscellaneous) Immunology and microbiology (all) Immunology and microbiology (miscellaneous) Neuroscience (all) Neuroscience (miscellaneous)
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: jvortega@iispv.cat elsa.maymo@urv.cat
    Author identifier: 0000-0002-6994-6115 0000-0002-9133-3120
    Record's date: 2025-02-01
    Paper version: info:eu-repo/semantics/publishedVersion
    Paper original source: Star Protocols. 4 (4): 102693-
    APA: Villanueva-Carmona, Teresa; Cedo, Lidia; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Vendrell, Joan; Fernandez-Veledo, Sonia (2023). Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice. Star Protocols, 4(4), 102693-. DOI: 10.1016/j.xpro.2023.102693
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications

  • Keywords:

    Biochemical Research Methods,Biochemistry, Genetics and Molecular Biology (Miscellaneous),Immunology and Microbiology (Miscellaneous),Neuroscience (Miscellaneous)
    Adipocytes
    Adipose tissue, white
    Animals
    Cell culture
    Cell isolation
    Humans
    Metabolism
    Mice
    Model organisms
    Biochemical research methods
    Biochemistry, genetics and molecular biology (all)
    Biochemistry, genetics and molecular biology (miscellaneous)
    Immunology and microbiology (all)
    Immunology and microbiology (miscellaneous)
    Neuroscience (all)
    Neuroscience (miscellaneous)

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