Autor según el artículo: López-Martínez, G. Margalef-Català, M. Salinas, F. Liti, G. Cordero-Otero, R.

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: LÓPEZ MARTÍNEZ, GEMA ISABEL MARGALEF CATALÀ, MARIA DEL MAR Salinas, F. Liti, G. CORDERO OTERO, RICARDO ROMÁN

Palabras clave: anhydrobiosis article ATG18 gene

Resumen: Recently, different dehydration-based technologies have been evaluated for the purpose of cell and tissue preservation. Although some early results have been promising, they have not satisfied the requirements for large-scale applications. The long experience of using quantitative trait loci (QTLs) with the yeast Saccharomyces cerevisiae has proven to be a good model organism for studying the link between complex phenotypes and DNA variations. Here, we use QTL analysis as a tool for identifying the specific yeast traits involved in dehydration stress tolerance. Three hybrids obtained from stable haploids and sequenced in the Saccharomyces Genome Resequencing Project showed intermediate dehydration tolerance in most cases. The dehydration resistance trait of 96 segregants from each hybrid was quantified. A smooth, continuous distribution of the anhydrobiosis tolerance trait was found, suggesting that this trait is determined by multiple QTLs. Therefore, we carried out a QTL analysis to identify the determinants of this dehydration tolerance trait at the genomic level. Among the genes identified after reciprocal hemizygosity assays, RSM22, ATG18 and DBR1 had not been referenced in previous studies. We report new phenotypes for these genes using a previously validated test. Finally, our data illustrates the power of this approach in the investigation of the complex cell dehydration phenotype. © 2015 López-Martínez et al.

Grupo de investigación: Biotecnologia Microbiana dels Aliments Biotecnologia Enològica

Áreas temáticas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1932-6203

Identificador del autor: n/a n/a n/a 0000-0002-2318-0775 0000-0001-5402-1278

Fecha de alta del registro: 2015-04-27

Volumen de revista: 10

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119606

DOI del artículo: 10.1371/journal.pone.0119606

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2015

Página inicial: 119606

Tipo de publicación: Article Artículo Article