Autor según el artículo: Maria del Carmen Portillo; Albert Mas

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: PORTILLO GUISADO, MARIA DEL CARMEN; MAS BARON, ALBERTO

Palabras clave: Fermentation High-throughput sequencing Wine

Resumen: Understanding the diversity and evolution of microorganisms during wine fermentation is essential for controlling its production. Previous studies have been primarily based on culture-dependent methods but recent incorporation of culture-independent molecular methods is showing a quite different view of microbial composition and diversity during the wine making process. Herein we applied barcoded pyrosequencing technology to monitor bacterial and yeast dynamics during laboratory scale spontaneous wine fermentation from Grenache variety. Members of the lactic acid bacteria (LAB) and acetic acid bacteria (AAB) were the most abundant, representing the orders Lactobacillales and Rhodospirillales more than 70% of the bacterial population. Other bacterial genera, not previously detected at the end of fermentation, were present in low proportion and their possible role remains unknown. Within the yeast community, the genera Hanseniaspora and Candida were dominant during the initial and mid fermentation while the final fermentation was mainly dominated by Candida and Saccharomyces. This study contributes to the knowledge of the microbial dynamics across spontaneous wine fermentation and presents high-throughput sequencing as a useful tool to monitor and evaluate bacterial and yeast diversity and dynamics during wine fermentation.

Grupo de investigación: Biotecnologia Enològica

Áreas temáticas: Enologia Enología Oenology

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0023-6438

Identificador del autor: 0000-0002-4576-0244; 0000-0002-0763-1679

Fecha de alta del registro: 2016-05-18

Página final: 321

Volumen de revista: 72

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://www.sciencedirect.com/science/article/pii/S002364381630264X?via%3Dihub

DOI del artículo: 10.1016/j.lwt.2016.05.009

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: 317

Tipo de publicación: Article Artículo Article