Autor según el artículo: Ricardo Closa- Monasterolo; Peter Rzehak; Richard Saffery; Eva Reischl; Marcela Covic; Simone Wahl; Veit Grote; Annick Xhonneux; Jean-Paul Langhendries; Natalia Ferre; Elvira Verduci; Enrica Riva; Piotr Socha; Dariusz Gruszfeld; Berthold Koletzko
Departamento: Medicina i Cirurgia
Autor/es de la URV: CLOSA MONASTEROLO, RICARDO; Peter Rzehak; Richard Saffery; Eva Reischl; Marcela Covic; Simone Wahl; Veit Grote; Annick Xhonneux; Jean-Paul Langhendries; Natalia Ferre; Elvira Verduci; Enrica Riva; Piotr Socha; Dariusz Gruszfeld; Berthold Koletzko
Palabras clave: Està en blanc
Resumen: Mounting evidence links prenatal exposure to maternal tobacco smoking with disruption of DNA methylation (DNAm) profile in the blood of infants. However, data on the postnatal stability of such DNAm signatures in childhood, as assessed by Epigenome Wide Association Studies (EWAS), are scarce. Objectives of this study were to investigate DNAm signatures associated with in utero tobacco smoke exposure beyond the 12th week of gestation in whole blood of children at age 5.5 years, to replicate previous findings in young European and American children and to assess their biological role by exploring databases and enrichment analysis. DNA methylation was measured in blood of 366 children of the multicentre European Childhood Obesity Project Study using the Illumina Infinium HM450 Beadchip (HM450K). An EWAS was conducted using linear regression of methylation values at each CpG site against in utero smoke exposure, adjusted for study characteristics, biological and technical effects. Methylation levels at five HM450K probes in MYO1G (cg12803068, cg22132788, cg19089201), CNTNAP2 (cg25949550), and FRMD4A (cg11813497) showed differential methylation that reached epigenome-wide significance according to the false-discovery-rate (FDR) criteria (q-value<0.05). Whereas cg25949550 showed decreased methylation (-2% DNAm β-value), increased methylation was observed for the other probes (9%: cg12803068; 5%: cg22132788; 4%: cg19089201 and 4%: cg11813497) in exposed relative to non-exposed subjects. This study thus replicates previous findings in children ages 3 to 5, 7 and 17 and confirms the postnatal stability of MYO1G, CNTNAP2 and FRMD4A differential methylation. The role of this differential methylation in mediating childhood phenotypes, previously associated with maternal smoking, requires further investigation.
Grupo de investigación: Unitat de Recerca en Pediatria, Nutrició i Desenvolupament Humà
Áreas temáticas: Ciències de la salut Ciencias de la salud Health sciences
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1932-6203
Identificador del autor: N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D
Fecha de alta del registro: 2016-06-10
Volumen de revista: 11
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0155554
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1371/journal.pone.0155554
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2016
Página inicial: Article number e0155554
Tipo de publicación: Article Artículo Article
Repositori URV