Autor según el artículo: Vladimir Baulin; The Hong Phong Nguyen; Vy T. H. Pham; Song Ha Nguyen; Rodney J. Croft; Brian Phillips; Russell J. Crawford; Elena P. Ivanova

Departamento: Enginyeria Química

Autor/es de la URV: BAULIN ., VLADIMIR; The Hong Phong Nguyen; Vy T. H. Pham; Song Ha Nguyen; Rodney J. Croft; Brian Phillips; Russell J. Crawford; Elena P. Ivanova

Resumen: The mechanisms by which various biological effects are triggered by exposure to an electromagnetic field are not fully understood and have been the subject of debate. Here, the effects of exposing typical representatives of the major microbial taxa to an 18 GHz microwave electromagnetic field (EMF)were studied. It appeared that the EMF exposure induced cell permeabilisation in all of the bacteria and yeast studied, while the cells remained viable (94% throughout the exposure), independent of the differences in cell membrane fatty acid and phospholipid composition. The resulting cell permeabilisation was confirmed by detection of the uptake of propidium iodine and 23 nm fluorescent silica nanospheres using transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). Upon EMF exposure, the bacterial cell membranes are believed to become permeable through quasi-endocytosis processes. The dosimetry analysis revealed that the EMF threshold level required to induce the uptake of the large (46 nm) nanopsheres was between three and six EMF doses, with a specific absorption rate (SAR) of 3 kW/kg and 5 kW/kg per exposure, respectively, depending on the bacterial taxa being studied. It is suggested that the taxonomic affiliation and lipid composition (e.g. the presence of phosphatidyl-glycerol and/or pentadecanoic fatty acid) may affect the extent of uptake of the large nanospheres (46 nm). Multiple 18 GHz EMF exposures over a one-hour period induced periodic anomalous increases in the cell growth behavior of two Staphylococcus aureus strains, namely ATCC 25923 and CIP 65.8T.

Grupo de investigación: Molecular simulation I: Complex Systems

Áreas temáticas: Chemical engineering Ingeniería química Enginyeria química

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1932-6203

Identificador del autor: 0000-0003-2086-4271; n/a; n/a; n/a; n/a; n/a; n/a; n/a

Fecha de alta del registro: 2016-09-13

Volumen de revista: 11

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0158135

DOI del artículo: 10.1371/journal.pone.0158135

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: Art.num. e158135

Tipo de publicación: Article Artículo Article