Autor según el artículo: Lluis Arola; Josep M del Bas; Antoni Caimari; Maria Isabel Rodriguez-Naranjo; Caroline E Childs; Carolina Paras Chavez; Annette L West; Elizabeth A Miles; Philip C Calder

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: AROLA FERRER, LUIS MARIA; Josep M del Bas; Antoni Caimari; Maria Isabel Rodriguez-Naranjo; Caroline E Childs; Carolina Paras Chavez; Annette L West; Elizabeth A Miles; Philip C Calder

Palabras clave: fatty liver disease fish oil inflammation

Resumen: Plasma lysophospholipids have emerged as signaling molecules with important effects on inflammation, insulin resistance, and fatty liver disease, each of which is linked closely to obesity. Dietary n-3 (ω-3) polyunsaturated fatty acids (PUFAs) may be able to improve these conditions. Objective: The objective of this study was to assess the response of plasma lysophospholipids to obesity, n-3 PUFA consumption, and a high-fat meal challenge to better understand the role of lysophospholipid metabolism in the progression of obesity-related disorders. Design: We determined the concentrations of 8 lysophosphatidylcholines, 11 lysophosphatidylethanolamines, and 7 lysophosphatidylinositols in the plasma of 34 normal-weight and 38 obese subjects randomly assigned to consume corn oil (control) or n-3 PUFA-rich fish oil (3 g/d; n = 15-19/group) for 90 d. Blood samples were collected on the last day of the study under fasting conditions and 6 h after a high-fat meal (1135 kcal, 86 g fat) challenge. The profile of secreted lysophospholipids was studied in HepG2 cells under palmitate-induced steatosis. Results: Obese and normal-weight subjects had different profiles of plasma lysophospholipids. A multivariate combination of the 26 lysophospholipids could discriminate between normal-weight and obese subjects with an accuracy of 98%. The high-fat meal challenge altered the concentration of plasma lysophosphatidylcholines in an oil treatment-dependent manner in normal-weight but not obese subjects, suggesting that obesity impairs the sensitivity of lysophospholipid metabolism to n-3 PUFAs. Noncytotoxic steatosis in HepG2 cells affected the secretion pattern of lysophospholipids, partially resembling the changes observed in the plasma of obese subjects. Conclusions: Obesity has a substantial impact on lysophospholipid metabolism, altering the plasma lysophospholipid profile and abolishing its sensitivity to dietary n-3 PUFAs. These effects could contribute to the onset or progression of alterations associated with obesity, such as inflammation, insulin resistance, and fatty liver disease.

Grupo de investigación: Grup de Recerca en Nutrigenòmica

Áreas temáticas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0002-9165

Identificador del autor: 0000-0003-2767-1974; 0000-0002-0700-2004; n/a; 0000-0003-2180-0974; 0000-0001-6832-224X; n/a; 0000-0002-3331-0684; 0000-0002-8643-0655; n/a

Fecha de alta del registro: 2016-09-19

Página final: 279

Volumen de revista: 104

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: http://ajcn.nutrition.org/content/104/2/266

DOI del artículo: 10.3945/ajcn.116.130872

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: 266

Tipo de publicación: Article Artículo Article